Introduction and purpose

Despite the various vaccination programs for protection against New Castle disease, it remains an important threat to the poultry industry. The ability of the probiotic bacteria to improve the immune system in both animals and humans supports their use as immune adjuvants for vaccination. The aim of the present study was to examine the effects of Lactobacillus acidophilus in ND vaccination.

Materials and methods

A total of 170 one day old chicks were divided in 5 groups. In groups A, B and C chicks were received L. acidophilus (5 × 10⁹, 3 × 10⁹ and 2 × 10⁹) and also vaccinated with inactivated and attenuated ND vaccines. In group D, chicks only vaccinated without bacterial inoculation and group E was negative control with neither vaccine nor bacteria. Then IgG and HI NDV antibody titers were measured in all tested groups.

Results

IgG and HI NDV antibody levels were significantly higher in Lactobacillus treated groups especially in group A with 5 × 10⁹ bacteria, than only vaccinated and negative control groups. Also antibody levels against NDV increased during the vaccination period especially in probiotic treated groups.

Conclusion

In conclusion, L. acidophilus can use for improving immunogenicity of NDV vaccination programs.

中文翻译：

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嗜酸乳杆菌对鸡新城疫疫苗的免疫增强作用。

介绍和目的

尽管有各种预防新城疫的疫苗接种计划，但这仍然是家禽业的重要威胁。益生菌改善动物和人类免疫系统的能力支持它们用作疫苗免疫佐剂。本研究的目的是检查嗜酸乳杆菌在ND疫苗接种中的作用。

材料和方法

将总共​​170只一天大的小鸡分成5组。在组A，B和C只小鸡接收嗜酸乳杆菌（5×10 ⁹，3×10 ⁹ 2×10 ⁹），并且还接种灭活和减毒ND疫苗。在D组中，只接种了未接种细菌的小鸡，E组是既没有疫苗也没有细菌的阴性对照。然后在所有测试组中测量IgG和HI NDV抗体滴度。

结果

乳杆菌治疗组中的IgG和HI NDV抗体水平显着高于仅接种疫苗和阴性对照组，尤其是具有5×10 ⁹细菌的A组。在疫苗接种期间，尤其是在益生菌治疗组中，抗NDV的抗体水平也增加。

结论

总之，嗜酸乳杆菌可用于改善NDV疫苗接种程序的免疫原性。