This study exploits dielectric spectroscopy to monitor the kinetics of red blood cells (RBC) storage lesions, focusing on those processes linked to cellular membrane interface known as β-dispersion. The dielectric response of RBC suspensions, exposed to blood-bank cold storage for 37 days, was studied using time-domain dielectric spectroscopy in the frequency range 500 kHz to 200 MHz. The measured dielectric processes are characterized by their dielectric strength (Δε) and their relaxation times (τ). Changes in the dielectric properties of the RBC suspensions, due to storage-related biophysical changes, were evaluated. For a quantitative characterization of RBC vitality, we characterized the shape of fresh and stored RBC and measured their deformability as expressed by their average elongation ratio, which was achieved under a shear stress of 3.0 Pa. During the second week of storage, an increment in the evolution of the relaxation times and in the dielectric permittivity strength of about 25% was observed. We propose that the characteristic increment of ATP, during the second and third weeks of storage, is responsible for the raise of the specific capacitance of cell membrane, which in turn explains the changes observed in the dielectric response when combined with the influence of the shape changes.

这项研究利用介电光谱技术监测红细胞（RBC）储存损伤的动力学，重点研究与细胞膜界面相关的过程，即β分散。使用时域介电谱在500 kHz至200 MHz的频率范围内研究了暴露于血库冷藏37天的RBC悬浮液的介电响应。所测量的介电过程的特征在于其介电强度（Δε）和弛豫时间（τ）。评估了由于存储相关的生物物理变化而引起的RBC悬浮液介电性能的变化。为了定量表征RBC的活力，我们表征了新鲜和储存的RBC的形状，并测量了它们的变形能力，用平均伸长率表示，这是在剪切应力为3时实现的。0 Pa。在存储的第二周，观察到松弛时间的演变和介电常数强度的增加约为25％。我们认为，在储存的第二和第三周内，ATP的特征增量是引起细胞膜比电容升高的原因，这反过来解释了当结合形状的影响时，介电响应的变化变化。