The effects of nitric oxide (NO) donor sodium nitroprusside (SNP) and various growth regulators on callus induction and shoot organogenesis in the commercially and pharmaceutically important medicinal plant Valeriana jatamansi is presented. Four different growth regulators at different concentrations were tested for their callusing efficiency followed by supplementation of SNP to the best responding growth regulator. Maximum callusing frequency (91.18%) was observed with the supplementation of 1.5 mg/L 1-Naphthaleneacetic acid + 15 μM SNP. 10% Coconut water (CW) was found to be the best growth regulator for the induction of shoots. The multiple shoot formation was also found to be enhanced with the supplementation of SNP. Maximum response for in vitro shoot multiplication was observed when the medium was supplemented with 10% CW + 15 μM SNP (89.32%) as compared to shoots induced in medium supplemented with 1.5 mg/L Benzyl aminopurine + 15 μM SNP (79.46%) respectively. No separate root induction medium was required as CW and CW + SNP supplementation was found to be sufficient enough to produce profuse rooting of the in vitro regenerated plants. Results revealed that SNP enhanced the callus and shoot induction frequencies. Supplementation of SNP also significantly reduced callus browing, allowed the tissues to recover and regenerate. This proposed in vitro culture system will be an effective mass propagation strategy for both commercial utilization and conservation of this highly valued plant species.

提出了一氧化氮（NO）供体硝普钠（SNP）和各种生长调节剂对商业和药学重要药用植物缬草（Valeriana jatamansi）愈伤组织诱导和芽器官发生的影响。测试了四个不同浓度的不同生长调节剂的愈伤功效，然后在响应最强的生长调节剂上添加了SNP。补充1.5 mg / L 1-萘乙酸+ 15μMSNP观察到最大的愈伤频率（91.18％）。发现10％椰子水（CW）是诱导芽的最佳生长调节剂。还发现补充SNP可以增强多次芽的形成。与分别添加1.5 mg / L苄基氨基嘌呤+ 15μMSNP（79.46％）的培养基诱导的芽相比，添加10％CW + 15μMSNP（89.32％）的培养基观察到了体外增殖的最大响应。 。不需要单独的根诱导培养基，因为发现CW和CW + SNP补充足以产生体外再生植物的大量生根。结果显示，SNP增强了愈伤组织和芽的诱导频率。补充SNP还可以显着减少愈伤组织的褐变，使组织得以恢复和再生。拟议的体外培养系统将是一种有效的大量繁殖策略，可用于商业利用和保护这种高价值植物。