A plant regeneration technique was successfully developed for the first time using young leaves harvested from two-y-old grafted seedlings in loquat (Eriobotrya japonica L.) cv. Dawuxing. Callus cultures were induced from the cut edge of the leaf explants after 2 wk of culture on Murashige and Skoog (MS) medium supplemented with 0.5 mg L⁻¹ 6-benzyladenine (BA) and 0.5 mg L⁻¹ 2,4-dichlorophenoxyacetic acid (2,4-D), in which callus cultures were induced at a frequency of 89.2%. White-greenish adventitious buds occurred after 3 wk of culture on MS medium supplemented with 0.8 mg L⁻¹ thidiazuron (TDZ), 0.3 mg L⁻¹ α-naphthaleneacetic acid (NAA), and 0.2 mg L⁻¹ AgNO₃, the adventitious buds developed to shoots after 5 wk of culture, with 30.6% of shoot induction rate. The shoots (about 5 cm in length with expanded leaves) rooted at a rate of 68.9% on the rooting medium consisting of ½ MS medium containing 1.0 mg L⁻¹ NAA and 1.0 mg L⁻¹ indolebutyric acid (IBA). The rooted plantlets, after hardening, survived satisfactorily in the transplantation matrix of peat and humus under optimum temperature, light, and water management. The morphology and inter simple sequence repeat (ISSR) marker banding patterns of the regenerated plants were all identical and similar to that of the mother plant. The plant regeneration technique developed in this study could be useful for Agrobacterium-mediated genetic transformation in loquat.

首次成功地使用了从two（Eriobotrya japonica L.）cv的两岁接枝幼苗中收获的嫩叶成功开发了一种植物再生技术。大五行 在补充0.5 mg L ^-1 6-苄基腺嘌呤（BA）和0.5 mg L ^-1 2,4-二氯苯氧基乙酸的Murashige和Skoog（MS）培养基上培养2周后，从叶片外植体的切缘诱导愈伤组织培养（2，4-D），其中愈伤组织培养物以89.2％的频率被诱导。在补充了0.8 mg L ^-1噻唑隆（TDZ），0.3 mg L ^-1α-萘乙酸（NAA）和0.2 mg L ^-1 AgNO _3的MS培养基上培养3周后，出现了白色绿色的不定芽。，不定芽在培养5周后发育成芽，芽诱导率为30.6％。芽（长约5cm，叶片扩张）在生根培养基上的生根率为68.9％，生根培养基由含有1.0 mg L ^-1 NAA和1.0 mg L ^-1吲哚丁酸（IBA）的1/2 MS培养基组成。生根的小苗变硬后，在最佳温度，光照和水分管理下，在泥炭和腐殖质的移植基质中可以令人满意地存活。再生植物的形态和内部简单序列重复（ISSR）标记条带模式均与母本植物相同且相似。本研究中开发的植物再生技术可用于农杆菌介导的lo的遗传转化。