In this study, we report a green synthesis of pharmaceutically active gold nanoparticles from marine red alga Acanthophora spicifera by the reduction of chloroauric acid. The formation of A. spicifera-mediated gold nanoparticles (As-AuNPs) was characterized by several analytical techniques. The crystalline and face-centered cubic (fcc) structure were confirmed by X-ray diffraction (XRD) analysis. Electron microscopy results confirmed that As-AuNPs were spherical and the average size of particles was < 20 nm. As-AuNPs hold a significant level of antioxidant activities than A. spicifera extract. As-AuNPs exhibited the highest antibacterial activity against Vibrio harveyi than Staphylococcus aureus at 100 µg/ml. Furthermore, As-AuNPs exhibited the utmost cytotoxicity against human colon adenocarcinoma (HT-29) cells and registered the half-maximal inhibitory concentration (IC₅₀) at 21.86 µg/ml. These findings authenticated that the synthesized As-AuNPs possess a broad spectrum of biological activities, and it can be effectively applied in the field of aquaculture and biomedical application.

在这项研究中，我们报告了通过还原氯金酸从海洋红藻Acanthophora spicifera 中绿色合成药物活性金纳米颗粒。A. spicifera介导的金纳米粒子 (As-AuNPs)的形成通过几种分析技术进行表征。通过 X 射线衍射 (XRD) 分析证实了晶体和面心立方 (fcc) 结构。电子显微镜结果证实 As-AuNPs 是球形的，颗粒的平均尺寸 < 20 nm。As-AuNPs 比A. spicifera提取物具有显着水平的抗氧化活性。As-AuNPs 对哈维弧菌的抗菌活性高于金黄色葡萄球菌100 微克/毫升。此外，As-AuNPs 对人结肠腺癌细胞 (HT-29) 表现出最大的细胞毒性，其半数抑制浓度 (IC ₅₀ ) 为 21.86 µg/ml。这些发现证明合成的As-AuNPs具有广泛的生物活性，可以有效地应用于水产养殖和生物医学领域。