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Bioconversion of coconut husk fibre through biorefinery process of alkaline pretreatment and enzymatic hydrolysis
Biomass Conversion and Biorefinery ( IF 3.5 ) Pub Date : 2020-07-18 , DOI: 10.1007/s13399-020-00895-8
Nur Akmal Solehah Din , Seng Joe Lim , Mohamad Yusof Maskat , Nurul Aqilah Mohd Zaini

Mature coconut husk (MCH) and young coconut husk (YCH) are abundantly generated as a by-product from coconut-based industry, which eventually lead to environmental pollution. However, the lignocellulose content in both types of coconut husk has the potential to be exploited as feedstock by way of biochemical processes through the concept of biorefinery. In this study, the MCH and YCH were subjected to alkaline pretreatment by sodium hydroxide (NaOH). Subsequently, enzymatic hydrolysis using Accellerase® 1500 was conducted to convert the lignocellulosic materials into fermentable sugars. The changes in surface morphology of MCH and YCH throughout the process were also observed. Alkaline pretreatment successfully removed 20.15 and 31.17% of lignin content in MCH and YCH, respectively. The increase in cellulose content after alkaline pretreatment was detected around 17% for MCH and 20% for YCH. For optimisation of enzymatic hydrolysis, 10% enzyme loading for 24 h showed significantly higher (p < 0.05) reducing sugar content in MCH and YCH hydrolysates of 19.17 g/L and 24.33 g/L, respectively. The efficiency of the hydrolysis process was proven by high sugar recovery of 71.23% for MCH and 89.49% for YCH. Concurrently, the morphological analysis showed the effect of the treatments on the surface of MCH and YCH, where the structure became swollen and collapsed as the majority of lignocellulosic materials were digested. Therefore, the study pointed out the potential of reutilising MCH and YCH as alternative resources for the future biochemical process feedstock.



中文翻译:

通过碱预处理和酶水解的生物精制工艺对椰子壳纤维进行生物转化

成熟的椰子壳(MCH)和年轻的椰子壳(YCH)作为椰子产业的副产品大量产生,最终导致环境污染。然而,两种类型的椰子壳中木质纤维素的含量都有可能通过生物精炼的概念通过生化过程被用作原料。在这项研究中,MCH和YCH用氢氧化钠(NaOH)进行了碱预处理。随后,使用Accellerase®1500进行酶水解,将木质纤维素材料转化为可发酵糖。在整个过程中,还观察到了MCH和YCH表面形态的变化。碱预处理分别成功去除了MCH和YCH中20.15和31.17%的木质素含量。检测到碱性预处理后纤维素含量的增加,MCH约为17%,YCH约为20%。为了优化酶促水解,在24小时内10%的酶负载量要显着提高(p <0.05)将MCH和YCH水解产物中的糖含量分别降低19.17 g / L和24.33 g / L。MCH和YCH的糖回收率分别为71.23%和89.49%,证明了水解过程的效率。同时,形态分析表明该处理对MCH和YCH的表面有影响,由于大部分木质纤维素材料被消化,结构变得肿胀和塌陷。因此,研究指出了将MCH和YCH用作未来生化过程原料的替代资源的潜力。

更新日期:2020-07-18
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