This study demonstrated the spin-coating of functional diblock copolymers to develop smart culture inserts for thermoresponsive cell adhesion/detachment control. One part of the block components, the poly(n-butyl methacrylate) block, strongly supported the water stable surface-immobilization of the thermoresponsive poly(N-isopropylacrylamide) (PNIPAAm) block, regardless of temperature. The chain length of the PNIPAAm blocks was varied to regulate thermal surface functions. Immobilized PNIPAAm concentrations became larger with increasing chain length (1.0–1.6 μg cm⁻²) and the thicknesses of individual layers were relatively comparable at 10-odd nanometers. A nanothin coating scarcely inhibited the permeability of the original porous membrane. When human fibroblasts were cultured on each surface at 37 °C, the efficiencies of cell adhesion and proliferation decreased with longer PNIPAAm chains. Meanwhile, by reducing the temperature to 20 °C, longer PNIPAAm chains promoted cell detachment owing to the significant thermoresponsive alteration of cell-surface affinity. Consequently, we successfully produced a favorable cell sheet by choosing an appropriate PNIPAAm length for block copolymers.

中文翻译：

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聚（N-异丙基丙烯酰胺）基嵌段共聚物在培养插入膜上的水稳定性纳米涂层，用于温度控制的细胞粘附。

这项研究证明了功能性双嵌段共聚物的旋涂技术可以开发出智能培养插入物，用于控制细胞的热响应/脱离。嵌段组分的一部分，即聚（甲基丙烯酸正丁酯）嵌段，无论温度如何，都强烈支持热敏性聚（N-异丙基丙烯酰胺）（PNIPAAm）嵌段的水稳定表面固定化。改变PNIPAAm嵌段的链长以调节热表面功能。固定的PNIPAAm浓度随着链长的增加而变大（1.0–1.6μgcm ^-2），并且各个层的厚度在10纳米以上相对可比。纳米薄涂层几乎不抑制原始多孔膜的渗透性。当在37°C的每个表面上培养人成纤维细胞时，PNIPAAm链越长，细胞粘附和增殖的效率越低。同时，通过将温度降低至20°C，更长的PNIPAAm链由于细胞表面亲和力的显着热响应性改变而促进了细胞脱离。因此，我们通过为嵌段共聚物选择合适的PNIPAAm长度，成功生产出了一种良好的电池片。