The transport of arsenic from soil through to edible crop is important when assessing the potential health risks from a food source. Samples of soil, irrigation water and the rice plant, Oryza sativa, were collected from an agricultural site in the Middle East. Total As, its speciation and DNA fractionation were evaluated using HPLC-ICP-MS in samples which included the root, stem, leaf and grain of the rice plant. Methodology was validated through the use of CRMs. The highest concentration of As was found in the root of the plant and the lowest in the grain. The concentrations found for As were: soil (aqua-regia extractable; 2.88 μg g⁻¹), irrigation water (0.58 μg L⁻¹), roots (8.28 μg g⁻¹), stem (4.00 μg g⁻¹), leaves (2.93 μg g⁻¹) and grain (1.02 μg g⁻¹). Levels of inorganic As^V and As^III were identified in the soil and plant material, while organo-arsenic species (DMA, MMA) were below the limit of detection. The ‘plant available’ levels of As in the soil (14%) were determined as part of a full, validated BCR three-step sequential extraction procedure. Since As can behave as a phosphate analogue, a method was developed for the extraction of vegetative DNA to determine the different forms of As associated with, or integrated within, the DNA fractions. Measurement of As in the DNA extracts were above the LOD (0.019 μg kg⁻¹) for the root, stem and leaf samples. The concentration of both weakly and strongly associated As with DNA obtained from the root, stem and leaf decreased with decreasing total As concentrations. A narrow, near-constant ratio for the strongly associated As value (As/total As DNA) in all root, stem and leaf DNA samples (41.3 ± 0.3%) was further evidence for the incorporation of As into the DNA.

中文翻译：

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水稻Oryza sativa中的砷形态及其DNA分离

在评估食物来源的潜在健康风险时，砷从土壤到食用作物的运输非常重要。土壤，灌溉水和水稻，样品水稻，从中东的农业网站收集。使用HPLC-ICP-MS对包括水稻植株的根，茎，叶和谷粒在内的样品中的总砷，其形态和DNA分级进行了评估。通过使用CRM验证了方法论。在植物的根部发现砷的浓度最高，而在谷粒中发现的砷浓度最低。As的浓度为：土壤（可提取的王水； 2.88μgg ^-1），灌溉水（0.58μgL ^-1），根（8.28μgg ^-1），茎（4.00μgg ^-1）。），叶片（2.93μgg ^-1）和谷物（1.02μgg ^-1）。在土壤和植物材料中鉴定出无机As ^V和As ^III的水平，而有机砷物种（DMA，MMA）低于检测极限。确定了土壤中砷的“植物有效”水平（14％），这是经过全面验证的BCR三步顺序萃取程序的一部分。由于As可以充当磷酸盐类似物，因此开发了一种提取营养性DNA的方法，以确定与DNA馏分相关或整合在其中的As的不同形式。DNA提取物中的As含量高于检测限（0.019μgkg ^-1）用于根，茎和叶样品。从根，茎和叶获得的DNA中弱结合和强结合的砷的浓度随总砷浓度的降低而降低。在所有根，茎和叶DNA样品中，强相关As值（As /总As DNA）的狭窄近恒定比率（41.3±0.3％）进一步证明了将As掺入DNA中。