当前位置: X-MOL 学术Analyst › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A multifunctional DNA nanostructure based on multicolor FRET for nuclease activity assay.
Analyst ( IF 3.6 ) Pub Date : 2020-07-17 , DOI: 10.1039/d0an01212b
Juan Hu 1 , Wen-Can Li , Jian-Ge Qiu , BingHua Jiang , Chun-Yang Zhang
Affiliation  

Nucleases play a crucial role in DNA replication, recombination and repair which are associated with cancers. Herein, we develop a four-color fluorescent probe for ratiometric detection of multiple nucleases. This four-color fluorescent probe consists of four fluorescent dyes connected by a DNA tetrahedral nanostructure with the involvement of multistep fluorescence resonance energy transfer (FRET). Based on the principle of self-assembly, the four-color fluorescent probe is constructed by integrating one acceptor with three spatially and spectrally distinct acceptors. A DNA tetrahedral nanostructure functions as a scaffold to link the acceptor dyes (i.e., diethylaminocoumarin (DEA), carboxyfluorescein (FAM), Texas Red, and Cy5). The fluorescence emissions of DEA, FAM, Texas Red and Cy5 can be observed through efficient multi-step energy transfer. This four-color fluorescent probe enables single excitation/four emissions, and it can be used for ratiometric detection of nucleases (i.e., XhoI, HindIII and KpnI) and the screening of nuclease inhibitors. Importantly, this four-color fluorescent probe can be further applied to discriminate multiple biomolecule targets by simply integrating the recognition sites of various biomolecules into the DNA tetrahedral nanostructure.

中文翻译:

基于多色FRET的多功能DNA纳米结构,用于核酸酶活性测定。

核酸酶在与癌症有关的DNA复制,重组和修复中起着至关重要的作用。在这里,我们开发了一种四色荧光探针,用于多种核酸酶的比例检测。这种四色荧光探针由通过四面体DNA纳米结构连接的四种荧光染料组成,涉及多步荧光共振能量转移(FRET)。基于自组装原理,通过将一个受体与三个在空间和光谱上不同的受体整合在一起,构建了四色荧光探针。DNA四面体纳米结构充当连接受体染料的支架(,二乙基氨基香豆素(DEA),羧基荧光素(FAM),德克萨斯红和Cy5)。DEA,FAM,Texas Red和Cy5的荧光发射可以通过高效的多步能量转移进行观察。这种四色荧光探针可实现单次激发/四次发射,可用于核酸酶(XhoI,HindIII和KpnI)的比例检测和核酸酶抑制剂的筛选。重要的是,通过将各种生物分子的识别位点简单地整合到DNA四面体纳米结构中,该四色荧光探针可以进一步用于区分多个生物分子靶标。
更新日期:2020-09-14
down
wechat
bug