Despite advances in wound treatment, wound-associated infections and delayed healing still remains an ‘unmet clinical need’. The present treatment modalities include topical application of ointments and perhaps it may better be substituted by phytochemical incorporated nanofibers which increases wound-healing efficiency and reduce risk of infections. Hence, the aim of this study was to synthesise Areca catechu–incorporated polycaprolactone scaffolds for wound-healing applications. In this study, the tender nut of Areca catechu plant was collected and extracted with ethanol using the maceration technique. The presence of various active phytochemical constituents of ethanolic fraction of Areca catechu like phenol, flavonoid, tannin and alkaloid were identified qualitatively and estimated quantitatively. Areca catechu incorporated 10%w/w polycaprolactone scaffolds were fabricated by electrospinning technique and characterised physico-chemically by Fourier-transform infrared spectroscopy and scanning electron microscope analysis. In vitro cytotoxicity analysis was evaluated with L929 fibroblasts and in vivo wound-healing studies using rat models for both polycaprolactone and Areca catechu–incorporated polycaprolactone scaffolds. Extract of Areca catechu exhibited antioxidant properties and antibacterial activity against Staphylococcus aureus and Psuedomonus aeruginosa. Scanning electron microscope image revealed the nanofibrous structural morphology of Areca catechu–incorporated polycaprolactone and polycaprolactone with average diameter of 350 and 399 nm, respectively. The characteristic peak of Fourier-transform infrared spectroscopy depicted the presence of biomolecules and detection of functional groups confirming the incorporation of Areca catechu into the polycaprolactone scaffold. Furthermore, cells were cytocompatible with 85% viability over Areca catechu–incorporated polycaprolactone scaffolds, and wounds treated with Areca catechu–incorporated polycaprolactone healed faster with a significant difference in the wound area than polycaprolactone controls. The phytochemical-incorporated polycaprolactone scaffolds with antioxidant, antimicrobial, biocompatible and wound-healing properties is proposed to be an indigenous approach towards wound care management globally and seems to be better and cost-effective wound dressings.

中文翻译：

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将植物化学物质掺入电纺支架中，用于体外和体内伤口愈合应用

尽管伤口治疗取得了进展，但伤口相关感染和愈合延迟仍然是“未满足的临床需求”。目前的治疗方式包括局部涂抹软膏，也许它可能更好地被植物化学掺入的纳米纤维替代，从而提高伤口愈合效率并降低感染风险。因此，本研究的目的是合成含有槟榔儿茶的聚己内酯支架，用于伤口愈合。在这项研究中，使用浸渍技术收集并用乙醇提取槟榔植物的嫩坚果。定性鉴定并定量评估槟榔儿茶乙醇部分的各种活性植物化学成分，如苯酚、黄酮类、单宁和生物碱。槟榔儿茶掺入 10%w/w 聚己内酯支架通过静电纺丝技术制造，并通过傅里叶变换红外光谱和扫描电子显微镜分析进行物理化学表征。使用 L929 成纤维细胞进行体外细胞毒性分析，并使用大鼠模型对聚己内酯和掺入槟榔儿茶的聚己内酯支架进行体内伤口愈合研究。槟榔提取物对金黄色葡萄球菌和铜绿假单胞菌具有抗氧化特性和抗菌活性。扫描电子显微镜图像显示了掺入槟榔儿茶的聚己内酯和聚己内酯的纳米纤维结构形态，平均直径分别为 350 和 399 nm。傅里叶变换红外光谱的特征峰描述了生物分子的存在和官能团的检测，证实了槟榔儿茶已掺入聚己内酯支架中。此外，与掺入槟榔儿茶的聚己内酯支架相比，细胞具有 85% 的细胞相容性，并且用掺入槟榔儿茶的聚己内酯治疗的伤口愈合得更快，伤口面积与聚己内酯对照组相比有显着差异。加入植物化学物质的聚己内酯支架具有抗氧化、抗菌、生物相容性和伤口愈合特性，被认为是全球伤口护理管理的本土方法，似乎是更好、更具成本效益的伤口敷料。