Cervical cancer is the fourth most common malignancy in women. The aim of this study was to investigate the functions of Ezrin in cervical cancer cells. Two cervical cancer cell lines, SiHa and CaSki, were cultured in vitro. Following the knockdown of Ezrin using siRNA, real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis were applied to analyze Ezrin expression at the messenger RNA (mRNA) and protein levels. Subsequently, wound healing assay, transwell assay, and sulforhodamine B (SRB) assay were used to detect the migration, invasion, and viability of cervical cancer cells, respectively. Results revealed that Ezrin siRNA can notably inhibit the migration and invasion of SiHa and CaSki cells (P < 0.05). However, knockdown of Ezrin shows no effects on the viability of SiHa and CaSki cells (P < 0.05). It is indicated that Ezrin plays a possible role in promoting the migration and invasion of cervical cancer cells and may be a therapeutic target to prevent metastasis of cervical cancer.

宫颈癌是女性中第四大最常见的恶性肿瘤。这项研究的目的是研究Ezrin在宫颈癌细胞中的功能。在体外培养了两种宫颈癌细胞系SiHa和CaSki。使用siRNA敲除Ezrin之后，应用实时逆转录聚合酶链反应（RT-PCR）和Western印迹分析来分析信使RNA（mRNA）和蛋白质水平的Ezrin表达。随后，伤口愈合测定，transwell测定和磺基罗丹明B（SRB）测定分别用于检测子宫颈癌细胞的迁移，侵袭和生存能力。结果表明，Ezrin siRNA可以显着抑制SiHa和CaSki细胞的迁移和侵袭（P  < 0.05）。但是，Ezrin的敲低对SiHa和CaSki细胞的存活率没有影响（P  <  0.05）。表明Ezrin在促进子宫颈癌细胞的迁移和侵袭中可能发挥作用，并且可能是预防子宫颈癌转移的治疗靶标。