Human umbilical cord mesenchymal stem cells (hUC-MSCs) are a promising alternative source of mesenchymal stem cells (MSCs) that are enormously attractive for clinical use. This study was designed to investigate the effect of recombinant human bone morphogenetic protein-7 (rhBMP-7) and/or osteogenic media (OMD) on bone regeneration of hUC-MSCs seeded on nanohydroxyapatite/collagen/poly(l-lactide) (nHAC/PLA) in a rabbit model. The characteristics of stem cells were analyzed by plastic adherence, cell phenotype, and multilineage differentiation potential. Cell proliferation was examined using cell counting kit-8 assay. Osteogenic differentiation was evaluated by quantitative Ca²⁺ concentration, PO₄³⁻ concentration, alkaline phosphatase (ALP) activity, osteocalcin (OCN) secretion, and mineralized matrix formation. Bone regeneration was investigated in jaw bone defect repair in rabbit by microcomputed tomography, fluorescent labeling, and hematoxylin and eosin staining. Except for initial stress response, OMD and OMD + rhBMP-7 inhibited the proliferation of hUC-MSCs seeded on nHAC/PLA; rhBMP-7 inhibited cell proliferation in the nonlogarithmic phase and attenuated the inhibitory effect of OMD on cell proliferation. The inhibitory effects of OMD, rhBMP-7, and OMD + rhBMP-7 on cell proliferation were ranked as OMD > OMD + rhBMP-7 > rhBMP-7. OMD, rhBMP-7, and OMD + rhBMP-7 promoted Ca²⁺ concentration, PO₄³⁻ concentration, ALP activity, OCN secretion, and mineralized matrix formation of hUC-MSCs seeded on nHAC/PLA. The promoting effects of OMD, rhBMP-7, and OMD+rhBMP-7 on Ca²⁺ concentration, PO₄³⁻ concentration, ALP activity, OCN secretion, and mineralized matrix formation were ranked as rhBMP-7 > OMD > OMD + rhBMP-7, OMD > OMD + rhBMP-7 > rhBMP-7, OMD > rhBMP-7 > OMD + rhBMP-7, rhBMP-7 > OMD + rhBMP-7 > OMD, and OMD > rhBMP-7 > OMD + rhBMP-7, respectively. In rabbit jaw bone defect repair, OMD, rhBMP-7, and OMD + rhBMP-7 enhanced bone regeneration of hUC-MSCs seeded on nHAC/PLA, but the largest bone mineral apposition rate and bone formation were presented in cultures with rhBMP-7. These findings suggested that the combined use of rhBMP-7 and OMD may have no ideal synergistic effect on bone regeneration of hUC-MSCs seeded on nHAC/PLA in rabbit jaw bone defect.

中文翻译：

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重组人 BMP-7 和成骨培养基的联合使用可能对接种在纳米羟基磷灰石/胶原蛋白/聚（l-丙交酯）上的人脐带间充质干细胞的 Leporine 骨再生没有理想的协同作用。

人脐带间充质干细胞 (hUC-MSCs) 是一种很有前途的间充质干细胞 (MSCs) 替代来源，对临床应用极具吸引力。本研究旨在研究重组人骨形态发生蛋白 7 (rhBMP-7) 和/或成骨培养基 (OMD) 对接种在纳米羟基磷灰石/胶原蛋白/聚 ( l-丙交酯) (nHAC ) 上的 hUC-MSCs 骨再生的影响。/PLA) 在兔子模型中。通过塑料粘附、细胞表型和多向分化潜能分析干细胞的特性。使用细胞计数试剂盒-8 测定法检查细胞增殖。通过定量 Ca ²⁺浓度、PO ₄ ³⁻评估成骨分化浓度、碱性磷酸酶 (ALP) 活性、骨钙素 (OCN) 分泌和矿化基质形成。通过显微计算机断层扫描、荧光标记和苏木精和伊红染色研究兔颌骨缺损修复中的骨再生。除初始应激反应外，OMD和OMD+rhBMP-7均抑制接种在nHAC/PLA上的hUC-MSCs的增殖；rhBMP-7在非对数期抑制细胞增殖并减弱OMD对细胞增殖的抑制作用。OMD、rhBMP-7、OMD+rhBMP-7对细胞增殖的抑制作用排列为OMD>OMD+rhBMP-7>rhBMP-7。OMD、rhBMP-7 和 OMD + rhBMP-7 促进 Ca ²⁺浓度，PO ₄ ³⁻接种在 nHAC/PLA 上的 hUC-MSC 的浓度、ALP 活性、OCN 分泌和矿化基质形成。OMD、rhBMP-7和OMD+rhBMP-7对Ca ²⁺浓度、PO ₄ ³⁻的促进作用浓度、ALP 活性、OCN 分泌和矿化基质形成排列为 rhBMP-7 > OMD > OMD + rhBMP-7，OMD > OMD + rhBMP-7 > rhBMP-7，OMD > rhBMP-7 > OMD + rhBMP-7 ，rhBMP-7 > OMD + rhBMP-7 > OMD，和 OMD > rhBMP-7 > OMD + rhBMP-7，分别。在兔颌骨缺损修复中，OMD、rhBMP-7 和 OMD + rhBMP-7 增强了接种在 nHAC/PLA 上的 hUC-MSC 的骨再生，但在含有 rhBMP-7 的培养物中呈现出最大的骨矿物质沉积率和骨形成. 这些发现表明，rhBMP-7 和 OMD 的联合使用可能对兔颌骨缺损中 nHAC/PLA 上接种的 hUC-MSCs 的骨再生没有理想的协同作用。