Purpose

This study aimed to explore the effect of GANT61 on regulating cell proliferation, cell apoptosis and cell cycle, and to investigate whether GANT61 would function in multiple myeloma (MM) via inhibiting Notch pathway. Methods: RPMI-8226 and U266 cells were treated by GANT61 (0, 2.5, 5.0, 10.0, 20.0, 30.0, 40.0, 50.0 μmol/L) for 18, 24 and 36 hours (h), and cell proliferation was detected by Cell Counting Kit 8. Then these cells were treated by GANT61 at 0, 2.5, 5.0, 10.0 μmol/L for 24 h or treated by 10.0 μmol/L GANT61 for 0, 18, 24 and 36 h, and cell apoptosis rate, apoptosis markers and cell cycle were detected by AV/PI, Western blot, and PI staining. Notch1, Jagged1, Jagged2 and Hes1 expressions were detected by qPCR and Western blot. Further rescue experiments were conducted by upregulating Notch1. Results: In RPMI-8226 and U266 cells, GANT61 inhibited cell proliferation, increased cell apoptosis rate and cell percentage of G1/G0 phase while decreased cell percentage of S phase in a dose- and time-dependent manner. Besides, GANT61 inhibited Notch1, Jagged1, Jagged2 and Hes1 expressions in a dose- and time-dependent manner as well. In rescue experiments, Notch1 upregulation attenuated the inhibition of cell proliferation, promotion of cell apoptosis, induction of G1/G0 cycle retardation and repression of Notch signaling pathway induced by GANT61 treatment in RPMI-8226 and U266 cells. Conclusions: GANT61 suppresses cell proliferation, promotes cell apoptosis and induces G1/G0 cycle retardation with a dose- and time-dependent manner through inhibiting Notch pathway in MM.

Abbreviations

MM: Multiple myeloma; Hh: Hedgehog; EMT: epithelial mesenchymal transition; AML: acute myeloid leukemia; GANT61: GLI antagonist; DMSO: dimethyl sulfoxide; CCK-8: Cell Counting Kit 8; C-Caspase 3: Cleaved Caspase 3; Bcl-2: B-cell lymphoma-2; RT-qPCR: real-time quantitative polymerase chain reaction; OD: optical density; PTCH1: Patched1

中文翻译：

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Gli 抑制剂 GANT61 通过抑制多发性骨髓瘤中的 Notch 通路，以剂量和时间依赖性方式抑制细胞增殖，促进细胞凋亡并诱导 G1/G0 周期延迟。

目的

本研究旨在探讨GANT61对细胞增殖、细胞凋亡和细胞周期的调节作用，并探讨GANT61是否通过抑制Notch通路在多发性骨髓瘤（MM）中发挥作用。方法: RPMI-8226和U266细胞分别用GANT61(0, 2.5, 5.0, 10.0, 20.0, 30.0, 40.0, 50.0 μmol/L)处理18, 24, 36 h (h), Cell检测细胞增殖情况Counting Kit 8. 然后这些细胞用0、2.5、5.0、10.0 μmol/L的GANT61处理24小时或用10.0 μmol/L的GANT61处理0、18、24和36小时，以及细胞凋亡率、凋亡标志物通过AV/PI、Western印迹和PI染色检测细胞周期和细胞周期。通过 qPCR 和蛋白质印迹检测 Notch1、Jagged1、Jagged2 和 Hes1 的表达。通过上调 Notch1 进行了进一步的拯救实验。结果：在RPMI-8226和U266细胞中，GANT61以剂量和时间依赖性方式抑制细胞增殖，增加细胞凋亡率和G1/G0期细胞百分比，同时降低S期细胞百分比。此外，GANT61 还以剂量和时间依赖性方式抑制 Notch1、Jagged1、Jagged2 和 Hes1 的表达。在拯救实验中，Notch1 上调减弱了 RPMI-8226 和 U266 细胞中 GANT61 处理诱导的细胞增殖抑制、细胞凋亡促进、G1/G0 周期延迟的诱导和 Notch 信号通路的抑制。结论：GANT61通过抑制MM的Notch通路，以剂量和时间依赖性方式抑制细胞增殖，促进细胞凋亡并诱导G1/G0周期延迟。

缩写

MM：多发性骨髓瘤；Hh：刺猬；EMT：上皮间质转化；AML：急性髓系白血病；GANT61：GLI 拮抗剂；DMSO：二甲亚砜；CCK-8：细胞计数试剂盒 8；C-Caspase 3：裂解的 Caspase 3；Bcl-2：B 细胞淋巴瘤-2；RT-qPCR：实时定量聚合酶链反应；OD：光密度；PTCH1：补丁1