Abstract

Hechtia glomerata, a Mexican medicinal plant employed against bacterial infections and as food, is taxonomically related to the genus Tillandsia which has anticancer activity. Organic and aqueous extracts of H. glomerata leaves were prepared and tested for cytotoxic and antibacterial activity. UPLC–QTOF–MS analysis determined the chemical composition of active extracts to find cytotoxic and antibacterial compounds. Hexane extract was cytotoxic against HepG2, Hep3B and MCF7 (IC₅₀: 24–28 µg/mL), whereas CHCl₃/MeOH extract against PC3 and MCF7 (IC₅₀: 25 and 32 µg/mL). CHCl₃/MeOH extract showed antibacterial activity against Staphylococcus aureus and Enterococcus faecium (MIC: 125 and 62.5 µg/mL). Hexane extract cytotoxic compounds were β-sitosterol, stigmasterol, phytol and ursolic acid. CHCl₃/MeOH extract antibacterial and/or cytotoxic compounds were daucosterol, oleanolic acid, resveratrol, quercetin, kaempferol, apigenin, cyanidin, p-coumaric acid and caffeic acid. This plant could be useful against bacterial infections and cancer. However, in vivo studies are needed to determine its toxicity and therapeutic efficacy.

摘要

Hechtia glomerata是一种墨西哥药用植物，用于对抗细菌感染和作为食物，在分类学上与具有抗癌活性的凤梨属有关。制备了H.glomerata叶子的有机和水提取物并测试其细胞毒性和抗菌活性。UPLC-QTOF-MS 分析确定了活性提取物的化学成分，以发现细胞毒性和抗菌化合物。己烷提取物对 HepG2、Hep3B 和 MCF7 具有细胞毒性（IC ₅₀：24–28 µg/mL），而 CHCl ₃ /MeOH 提取物对 PC3 和 MCF7具有细胞毒性（IC _50：25和 32 µg/mL）。CHCl _{3 /MeOH 提取物对}金黄色葡萄球菌具有抗菌活性和粪肠球菌（MIC：125 和 62.5 µg/mL）。己烷提取物的细胞毒性化合物为β-谷甾醇、豆甾醇、植醇和熊果酸。CHCl ₃ /MeOH 提取物的抗菌和/或细胞毒性化合物是胡萝卜甾醇、齐墩果酸、白藜芦醇、槲皮素、山柰酚、芹菜素、花青素、对香豆酸和咖啡酸。这种植物可能对细菌感染和癌症有用。然而，需要进行体内研究来确定其毒性和治疗效果。