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TOR kinase activity in Chlamydomonas reinhardtii is modulated by cellular metabolic states
FEBS Letters ( IF 3.0 ) Pub Date : 2020-08-03 , DOI: 10.1002/1873-3468.13888
Shivani Upadhyaya 1 , Shreya Agrawal 2 , Anmol Gorakshakar 3 , Basuthkar Jagadeeshwar Rao 4
Affiliation  

Target of rapamycin (TOR) kinase is a sensor and a central integrator of internal and external metabolic cues. However, in algae and in higher plants, the components of TOR kinase signaling are yet to be characterized. Here, we establish an assay system to study TOR kinase activity in Chlamydomonas reinhardtii using the phosphorylation status of its putative downstream target, CrS6K. Using this assay, we probe the modulation of cellular TOR kinase activity under various physiological states such as photoautotrophy, heterotrophy, mixotrophy, and nitrogen (N) starvation. Importantly, we uncover that excess acetate in the medium leads to high cellular reactive oxygen species levels, triggering autophagy and a concomitant drop in TOR kinase activity in a dose‐dependent manner, thus leading to a N‐starvation‐like cellular phenotype, even when nitrogen is present.

中文翻译:

莱茵衣藻中的 TOR 激酶活性受细胞代谢状态的调节

雷帕霉素 (TOR) 激酶的靶标是一种传感器,也是内部和外部代谢信号的中央整合器。然而,在藻类和高等植物中,TOR 激酶信号传导的成分尚未得到表征。在这里,我们建立了一个测定系统,使用其假定的下游靶标 CrS6K 的磷酸化状态研究莱茵衣藻中的 TOR 激酶活性。使用这种测定,我们探测了在各种生理状态下细胞 TOR 激酶活性的调节,如光能自养、异养、混合营养和氮 (N) 饥饿。重要的是,我们发现培养基中过量的醋酸盐会导致细胞活性氧水平升高,从而引发自噬,并以剂量​​依赖性方式同时降低 TOR 激酶活性,从而导致类似 N 饥饿的细胞表型,
更新日期:2020-08-03
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