Mitochondria are necessary for the transition from oocyte to embryo and for early embryonic development. Mitofusin 1 is the main mediator of mitochondrial fusion and homeostasis. We investigated Mitofusin 1 expression levels in porcine somatic cell nuclear transfer (SCNT) embryos. The rate of blastocyst formation in SCNT embryos was reduced significantly compared with that of parthenogenetic activation embryos. SCNT embryos showed significantly decreased Mitofusin 1 expression and mitochondrial membrane potential, while exhibiting increased reactive oxygen species and apoptosis. Mitochondrial functional changes were observed in the SCNT embryos and may be correlated with low levels of Mitofusin 1 to negatively affect development.

中文翻译：

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mitofusin 1 的低表达与猪体细胞核移植胚胎中的线粒体功能障碍和细胞凋亡有关。

线粒体对于从卵母细胞到胚胎的转变以及早期胚胎发育是必需的。Mitofusin 1 是线粒体融合和体内平衡的主要介质。我们研究了猪体细胞核移植 (SCNT) 胚胎中 Mitofusin 1 的表达水平。与孤雌激活胚胎相比，SCNT 胚胎囊胚形成率显着降低。SCNT 胚胎显示 Mitofusin 1 表达和线粒体膜电位显着降低，同时表现出活性氧和细胞凋亡增加。在 SCNT 胚胎中观察到线粒体功能变化，并且可能与低水平的 Mitofusin 1 相关，从而对发育产生负面影响。