Nowadays great deal of research is physiological field is conducted on experimental animals and there is a lot of criticism from the wide public on methods used. Therefore, recently there is a lot of effort focused on the welfare of the animals. Main aim of this study is to determine the effect of experimental sample collection method on the selected parameters of stress. In the experiment two sample collections of rabbit blood from marginal ear vein were realized – first using standard method with one person fixing the animal and other collecting the blood using gently fixating the animal. In the second groups experimental method of inserting the experimental animal into a sack and further collection in dark was realized. During the experiment the levels of cortisol – main stress indicator in organism and other health parameters of animals including mineral profile and haematological parameters were observed. Our results show no significant changes in levels of cortisol but also a decreasing tendency in the sample from the second (dark) collection. Haematological parameters were generally in the reference values and any significant changes except levels of lymphocytes and percent of lymphocytes which shown significant increase in the second collection period were found. Also the levels of mean corpuscular haemoglobin and percent of neutrophils unveiled a significant decrease in values. Values of mineral profile parameters have indicated no significant changes except the levels of phosphorus. Based on the result we can state that the experimental sample collection has no effect on blood parameters of the animals but we spectated a statistically insignificant decrease in the levels of cortisol which can suggest that the dark collection is possibly less stressful to the animals.

如今，生理学领域的大量研究都是在实验动物上进行的，而公众对所使用的方法也有很多批评。因此，最近人们在动物福利方面做出了很多努力。本研究的主要目的是确定实验样本收集方法对所选应力参数的影响。在实验中，实现了从耳缘静脉采集兔血液的两次样本——第一个使用标准方法，一个人固定动物，第二个使用轻轻固定动物来收集血液。在第二组中，实现了将实验动物插入麻袋并在黑暗中进一步收集的实验方法。在实验过程中，观察了机体主要应激指标皮质醇的水平以及动物的其他健康参数，包括矿物质成分和血液学参数。我们的结果显示，皮质醇水平没有显着变化，但第二次（暗）采集的样品中皮质醇水平有下降趋势。血液学参数基本在参考值范围内，除了淋巴细胞水平和淋巴细胞百分比在第二个采集期显着增加外，未发现任何显着变化。此外，平均红细胞血红蛋白水平和中性粒细胞百分比的数值也显着下降。矿物剖面参数值表明除磷含量外没有显着变化。根据结果​​，我们可以说，实验样品采集对动物的血液参数没有影响，但我们观察到皮质醇水平在统计上没有显着下降，这表明暗采集可能对动物的压力较小。