Larimichthys polyactis is an economically important species in the Northwestern Pacific. In this study, characters of candidate microsatellite markers for L. polyactis were profiled and 24 polymorphic loci were developed using high-throughput sequencing method. A total of 6.085G raw data was obtained, and 7490 microsatellite-rich fragments were examined. Among them, 4199 dinucleotide, 2519 trinucleotide, 625 tetranucleotide, 121 pentanucleotide and 26 hexanucleotide repeats were detected. Besides, 24 candidate polymorphic microsatellite primers were isolated and characterized. The polymorphism information content of these microsatellite markers was 0.582 to 0.796, and all loci were highly polymorphic (PIC>0.50). The results of this study can not only provide abundant markers for the further genetic research of L. polyactis, but also provide basic information for understanding its distribution characteristics of microsatellites.

Larimichthys polyactis是西北太平洋的一个重要经济物种。在这项研究中，描绘了多形乳杆菌候选微卫星标记的特征，并使用高通量测序方法开发了24个多态位点。总共获得了6.085G的原始数据，并检查了7490个富含微卫星的碎片。其中，检测到4199个二核苷酸，2519个三核苷酸，625个四核苷酸，121个五核苷酸和26个六核苷酸重复。此外，分离并鉴定了24种候选多态性微卫星引物。这些微卫星标记的多态性信息含量为0.582至0.796，所有位点均为高度多态性（PIC> 0.50）。本研究结果不仅为进一步研究多act.Lacts提供了丰富的标记，而且为了解其微卫星的分布特征提供了基础信息。