Abstract ‘Nanguo’ pears are prone to core browning at ambient temperatures. To reveal the role of membrane lipid metabolism in the process of core browning, fruit were treated with n-butanol, a phospholipase D (PLD) inhibitor. Changes in phospholipids and fatty acids, as well as the activities and gene expression of key enzymes were analyzed. Membrane phenotype and ultrastructure were also documented. Phospholipid and fatty acid levels, the activities of PLD and lipoxygenase (LOX), and gene expression changed during fruit ripening. PLD activity and gene expression in n-butanol-treated fruit were lower than those in control fruit, and the symptoms of core browning appeared later and were less severe. Furthermore, the membrane ultrastructure of fruit treated with n-butanol was intact compared with that in control fruit. These results suggested that membrane lipid metabolism plays an important role in the core browning of harvested ‘Nanguo’ pears, and that n-butanol treatment can alleviate disorder development by regulating membrane lipid metabolism.

中文翻译：

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“南果”梨常温贮藏过程中膜脂代谢与果核褐变的关系

摘要 '南果'梨在常温下易发生核褐变。为了揭示膜脂代谢在核心褐变过程中的作用，用正丁醇（一种磷脂酶 D (PLD) 抑制剂）处理水果。分析了磷脂和脂肪酸的变化，以及关键酶的活性和基因表达。还记录了膜表型和超微结构。磷脂和脂肪酸水平、PLD 和脂氧合酶 (LOX) 的活性以及基因表达在果实成熟过程中发生了变化。正丁醇处理果实的PLD活性和基因表达量低于对照果实，核心褐变症状出现较晚且较轻。此外，与对照水果相比，用正丁醇处理的水果的膜超微结构是完整的。