Target capture DNA library preparation methods primarily use the biotin-streptavidin interaction to enrich target DNA, requiring complex operations and a time-consuming workflow with a series of wash steps at regulated temperatures. Here, a new method is presented termed 'hook ligation' for target DNA library preparation, using CircLigase to capture target DNA. The concept was validated by library preparation, sequencing, and acquisition of target DNA fragment information through bioinformatics analysis. An efficient reference point for single-strand DNA ligation to a hook sequence using CircLigase is also provided and it is shown that the efficiency can be influenced by the length and the position of the complementary area on the target DNA.

中文翻译：

---

使用 CircLigase 介导的钩连接的新型目标捕获 DNA 文库制备方法

目标捕获 DNA 文库制备方法主要使用生物素-链霉亲和素相互作用来富集目标 DNA，需要复杂的操作和耗时的工作流程，并在调节温度下进行一系列洗涤步骤。在这里，提出了一种称为“钩连接”的新方法，用于制备目标 DNA 文库，使用 CircLigase 捕获目标 DNA。该概念通过文库制备、测序和通过生物信息学分析获取目标 DNA 片段信息得到验证。还提供了使用 CircLigase 将单链 DNA 连接到钩子序列的有效参考点，并且表明效率会受到目标 DNA 上互补区域的长度和位置的影响。