Background

Baicalin has many biological properties such as anti-oxidation and anti-allergy. The current study aimed to explore the effect of Baicalin on allergic rhinitis (AR) and its potential mechanism of action.

Methods

Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation. Expression levels of Th17 and Treg cells-related proteins in nasal mucosa and peripheral blood cells were detected by real-time quantitative PCR, flow cytometry and Western blot. The mice were randomly divided into Control, ovalbumin (OVA), l-Baicalin, H-Baicalin, DSGC, 3-MA, and H-Baicalin + Rapa groups. Changes of allergic rhinitis conditions and eosinophil infiltration of the mice were detected and scored by Diff-Quik staining, and histological changes were observed by Hematoxylin & Eosin (H&E) staining and Periodate Schiff (PAS) staining. Serological changes, expression levels of interleukin-17A (IL-17A), interleukin-10 (IL-10), eosinophilic cationic protein (ECP) and anti-OVA-specific antibodies were detected by Enzyme-linked immunosorbent assay (ELISA).

Results

Clinical case analysis found that AR patients had a Th17/Treg imbalance and activated autophagy, however, Baicalin restored Th17/Treg cell balance and inhibited autophagy in vitro. in vivo experiments demonstrated that Baicalin inhibited OVA-induced allergic nasal symptoms and the activation of autophagy pathway, which was the same as the regulation of 3-MA, while Rapa could weaken the effects of H-baicalin. Moreover, Baicalin reduced the infiltration of different inflammatory cells of the nasal lavage fluid, prevented the damages to epithelial cells, and improved nasal mucosal thickness and mucus secretion. In addition, Baicalin regulated the balance of mouse anti-OVA-specific antibody levels and expressions of Th17/Treg-associated cytokines.

Conclusion

Our study revealed that Baicalin can be used to treat AR, and the effect is realized through inhibiting autophagy to regulate Th17/Treg cell differentiation.

中文翻译：

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黄ical苷通过抑制变应性鼻炎中的自噬来调节Treg / Th17细胞失衡。

背景

黄ical苷具有许多生物特性，例如抗氧化和抗过敏。目前的研究旨在探讨黄ical苷对过敏性鼻炎（AR）的作用及其潜在的作用机制。

方法

通过密度梯度离心分离外周血单个核细胞（PBMC）。实时定量PCR，流式细胞仪和Western blot检测鼻粘膜和外周血细胞中Th17和Treg细胞相关蛋白的表达水平。将小鼠随机分为对照组，卵清蛋白（OVA），l-黄ical苷，H-黄ical苷，DSGC，3-MA和H-黄ical苷+ Rapa组。通过Diff-Quik染色检测变应性鼻炎状况的变化和嗜酸性粒细胞浸润的评分，并通过苏木精和曙红（H＆E）染色和高碘酸盐Schiff（PAS）染色观察组织学变化。通过酶联免疫吸附试验（ELISA）检测血清学变化，白介素17A（IL-17A），白介素10（IL-10），嗜酸性阳离子蛋白（ECP）和抗OVA特异性抗体。

结果

临床病例分析发现，AR患者有Th17 / Treg失衡和自噬激活，但是，黄ical苷在体外能恢复Th17 / Treg细胞平衡并抑制自噬。体内实验表明，黄ical苷能抑制OVA诱导的过敏性鼻部症状和自噬途径的激活，与3-MA的调节相同，而Rapa可以减弱H-黄ical苷的作用。此外，黄ical苷减少了鼻灌洗液中不同炎症细胞的浸润，防止了对上皮细胞的损害，并改善了鼻粘膜厚度和粘液分泌。此外，黄ical苷还调节小鼠抗OVA特异性抗体水平和Th17 / Treg相关细胞因子表达的平衡。

结论

我们的研究表明，黄in苷可用于治疗AR，其作用是通过抑制自噬来调节Th17 / Treg细胞分化来实现的。