Infectious bronchitis (IB) is a viral infection of the chicken respiratory tract that causes substantial economic burden on the industry. Simple, specific and rapid diagnosis of this disease is critical for the initiation of appropriate control measures. Conventional molecular diagnostic methods require a relatively sophisticated equipment and skilled staff. Here we describe a rapid, simple, semi-quantative, closed-tube, single-step, real-time- reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for IB and compare our assay with quantative, reverse transcription- polymerase chain reaction (RT-qPCR). The limit of detection (LOD) of our RT-LAMP assay is 1 EID₅₀/ ml. Clinical evaluation of samples from diseased chickens with our RT-LAMP showed a very good concordance with RT-qPCR. Our assay enables simple, specific, rapid molecular detection and semi-quantification of the infectious bronchitis virus (IBV) in veterinary diagnostic laboratories. Furthermore, our RT-LAMP detection is carried out in a sealed tube, eliminating the risk of false-positive results in subsequent tests because of any contamination of the work area as in the case of lateral flow strip or gel electrophoresis-based amplicon detection.

传染性支气管炎（IB）是鸡呼吸道的病毒感染，对行业造成巨大的经济负担。对这种疾病的简单，特异性和快速诊断对于采取适当的控制措施至关重要。常规的分子诊断方法需要相对复杂的设备和熟练的人员。在这里，我们描述了一种针对IB的快速，简单，半定量，闭管，单步，实时逆转录-环介导的等温扩增（RT-LAMP）分析，并将我们的分析与定量，逆转录-聚合酶链反应（RT-qPCR）。我们的RT-LAMP分析的检测极限（LOD）为1 EID ₅₀/毫升。用我们的RT-LAMP对患病鸡的样品进行的临床评估显示，与RT-qPCR有很好的一致性。我们的检测方法可在兽医诊断实验室中对传染性支气管炎病毒（IBV）进行简单，特异性，快速的分子检测和半定量。此外，我们的RT-LAMP检测在密封的试管中进行，从而消除了在后续测试中由于工作区的任何污染而导致的假阳性结果的风险，例如基于横向流条或基于凝胶电泳的扩增子检测。