Abstract Cross-linked enzyme aggregate (CLEA) microcapsules of extracellular enzymes released by lichen Dermatocarpon vellereceum was examined for dye removal from textile wastewater. CLEA-microcapsules showed efficient decolorization of Brilliant Blue R (BBR), Direct Red 2B, Brilliant Blue GRL, Green HE4B, Red M5B, Blue 2RNL, Reactive Orange 3R, Yellow HE4G and Orange 2RX. Maximum decolorization (82%) of BBR was observed at optimum conditions (40 °C, pH 8, and 1.5 mM H2O2). Encapsulation efficiency (EE%) of lignin peroxidase (LiP) and manganese peroxidase (MnP) was calculated as 34.85 and 27.12%, respectively. Percent recycling efficiency (RE%) of LiP after BBR exposure was observed as 84, 72, 56 and 36% after 2nd, 3rd, 4th and 5th cycle, respectively, whereas, RE% of MnP after BBR exposure was 84, 69, 54 and 40%, respectively. Activity of LiP was retained up to 42% even after 60 days of storage, however, MnP was retained up to 36%. Central composite design of response surface methodology for CLEA-microcapsules mediated decolorization showed good correlation between predicted and observed values. BBR was cleaved to form N-ethyl-N-[(3-sulfophrnyl)methyl]pentan-3-iminium; 2-ethenyl-3-methyl-1,4-dihydronapthalene; 3-(aminomethyl)benzene-1-sulphonic acid; penta-1,4-diene and N-ethyl-2-methyl-penta-2,4-dien-1-amine, that showed significant reduction in phytotoxicity and genotoxicity. Altogether, these results suggest that CLEA-microcapsules could be utilized for efficient removal of dyes from textile effluents.

中文翻译：

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增强应用地衣 Dermatocarpon vellereceum 的交联酶聚集体释放胞外酶降解纺织染料

摘要 研究了由 Dermatocarpon vellereceum 释放的细胞外酶的交联酶聚集体 (CLEA) 微胶囊从纺织废水中去除染料。CLEA 微胶囊对亮蓝 R (BBR)、直接红 2B、亮蓝 GRL、绿色 HE4B、红色 M5B、蓝色 2RNL、活性橙 3R、黄色 HE4G 和橙色 2RX 显示出有效的脱色。在最佳条件（40 °C、pH 8 和 1.5 mM H2O2）下观察到 BBR 的最大脱色 (82%)。木质素过氧化物酶 (LiP) 和锰过氧化物酶 (MnP) 的包封率 (EE%) 分别计算为 34.85 和 27.12%。在第 2、3、4 和第 5 次循环后，BBR 暴露后 LiP 的回收效率百分比 (RE%) 分别为 84、72、56 和 36%，而 BBR 暴露后 MnP 的 RE% 为 84、69、54和 40%，分别。即使在储存 60 天后，LiP 的活性仍保持高达 42%，然而，MnP 的活性保持高达 36%。CLEA-微胶囊介导的脱色响应面方法的中心复合设计显示预测值和观察值之间具有良好的相关性。BBR被裂解形成N-乙基-N-[(3-磺基)甲基]pentan-3-iminium；2-乙烯基-3-甲基-1,4-二氢萘；3-(氨基甲基)苯-1-磺酸；penta-1,4-diene 和 N-ethyl-2-methyl-penta-2,4-dien-1-amine，显示出显着降低植物毒性和遗传毒性。总之，这些结果表明 CLEA 微胶囊可用于有效去除纺织废水中的染料。CLEA-微胶囊介导的脱色响应面方法的中心复合设计显示预测值和观察值之间具有良好的相关性。BBR被裂解形成N-乙基-N-[(3-磺基)甲基]pentan-3-iminium；2-乙烯基-3-甲基-1,4-二氢萘；3-(氨基甲基)苯-1-磺酸；penta-1,4-diene 和 N-ethyl-2-methyl-penta-2,4-dien-1-amine，显示出显着降低植物毒性和遗传毒性。总之，这些结果表明 CLEA 微胶囊可用于有效去除纺织废水中的染料。CLEA-微胶囊介导的脱色响应面方法的中心复合设计显示预测值和观察值之间具有良好的相关性。BBR被裂解形成N-乙基-N-[(3-磺基)甲基]pentan-3-iminium；2-乙烯基-3-甲基-1,4-二氢萘；3-(氨基甲基)苯-1-磺酸；penta-1,4-diene 和 N-ethyl-2-methyl-penta-2,4-dien-1-amine，显示出显着降低植物毒性和遗传毒性。总之，这些结果表明 CLEA 微胶囊可用于有效去除纺织废水中的染料。4-diene 和 N-ethyl-2-methyl-penta-2,4-dien-1-amine，显示出显着降低植物毒性和遗传毒性。总之，这些结果表明 CLEA 微胶囊可用于有效去除纺织废水中的染料。4-diene 和 N-ethyl-2-methyl-penta-2,4-dien-1-amine，显示出显着降低植物毒性和遗传毒性。总之，这些结果表明 CLEA 微胶囊可用于有效去除纺织废水中的染料。