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Gonadal analysis in the F1 progeny of a gynogenetic Siberian sturgeon Acipenser baerii female.
Animal Reproduction Science ( IF 2.2 ) Pub Date : 2020-07-17 , DOI: 10.1016/j.anireprosci.2020.106548
D Fopp-Bayat 1 , J Szadkowska 1 , M Szczepkowski 2 , B Szczepkowska 2 , K Naumowicz 3
Affiliation  

This is the first report of reproduction of a gynogenetic female Siberian sturgeon (Acipenser baerii) and subsequent production of viable offspring with differentiated gonads. Gonads were sampled twice from 30 randomly selected individuals. Gonadal fragments were examined histologically to identify the sex ratio and the sexual maturation stage. The first examined group consisted of 20 % females, 45 % males and 35 % sexually undifferentiated individuals, whereas the second group was composed of 30 % females and 70 % males. The average gonadosomatic index was determined to be 2.72 % and 2.80 % in the first and second groups, respectively. Molecular analysis based on five microsatellite DNA loci was conducted for all the fish for which there were evaluations. The aim of the study was to determine whether the gynogenetic Siberian sturgeon female that produced the offspring that were evaluated was a “superfemale” (WW) or a normal female (ZW). A “superfemale”, therefore, is a female of the WW genotype that will produce only females when mated to a male with a ZZ genotype. The presence of female and male offspring clearly indicates that the Siberian sturgeon female that produced the offspring was not a “superfemale”.



中文翻译:

雌性西伯利亚鲟鱼 Acipenser baerii 雌性 F1 后代的性腺分析。

这是关于雌性西伯利亚鲟( Acipenser baerii)繁殖的第一份报告) 并随后产生具有分化性腺的可行后代。从 30 个随机选择的个体中对性腺进行两次采样。对性腺片段进行组织学检查以确定性别比例和性成熟阶段。第一组由 20% 的女性、45% 的男性和 35% 的未分化个体组成,而第二组由 30% 的女性和 70% 的男性组成。第一组和第二组的平均性腺体指数分别确定为 2.72% 和 2.80%。对所有进行评估的鱼进行了基于五个微卫星 DNA 基因座的分子分析。该研究的目的是确定产生被评估后代的雌性西伯利亚鲟鱼雌性是“超雌性”(WW)还是正常雌性(ZW)。因此,“超级雌性”是 WW 基因型的雌性,与具有 ZZ 基因型的雄性交配时只会产生雌性。雌雄后代的存在清楚地表明,产生后代的雌性西伯利亚鲟鱼并不是“超级雌性”。

更新日期:2020-07-23
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