The goal of this study was to identify and characterize effects of repeated estrous synchronization (ES) treatments on the regulation of ovarian intergenic long non-coding RNAs (lincRNAs) in dairy goats. Six does were randomly assigned to a group administered three ES treatment regimens separated by 2 weeks or to a group administered only one ES treatment regimen (control) at the same time as the third ES treatment in the does administered the three hormonal regimens for ES. The paired-end RNA Sequencing procedures were used to evaluate lincRNAs of ovarian tissues. A total of 134 lincRNAs were differentially abundant between the two treatment groups. Several target genes were annotated and were related to hormone activity, cellular response to hormone stimulus, response to hormone, female pregnancy, as well as regulation of hormone secretion. These genes were noticeably enriched in MAPK, Hippo, estrogen signaling pathways, oocyte meiosis, progesterone-mediated oocyte maturation, ovarian steroidogenesis as well as GnRH signaling pathways. According to the enriched GO terms and KEGG pathways of regulated genes, 13 differentially abundant lincRNAs could be promising candidates for regulating reproductive functions of female goats. Current results indicate that repeated treatments with gonadotropins affected hormone sensitivity, estrogen synthesis, and ovarian function. The results also indicated that when there was imposing of the three hormonal treatment regimens for ES, there were several lincRNAs that could contribute to dysregulation of several genes that are important for reproduction in dairy goats. Findings provide novel insights for further investigation of lncRNAs biological functions in goats.

本研究的目的是确定和表征重复发情同步 (ES) 治疗对奶山羊卵巢基因间长链非编码 RNA (lincRNA) 调节的影响。将六个剂量随机分配到施用三个 ES 治疗方案的组，该组相隔 2 周，或分配给仅施用一种 ES 治疗方案（对照）的组，其中在施用了三种激素方案的 ES 的剂量中进行第三次 ES 治疗。双端RNA测序程序用于评估卵巢组织的lincRNA。两个治疗组之间共有 134 种 lincRNA 的丰度不同。注释了几个靶基因，它们与激素活性、细胞对激素刺激的反应、对激素的反应、女性怀孕以及激素分泌的调节有关。这些基因在 MAPK、Hippo、雌激素信号通路、卵母细胞减数分裂、孕酮介导的卵母细胞成熟、卵巢类固醇生成以及 GnRH 信号通路中明显富集。根据富集的 GO 术语和受调控基因的 KEGG 通路，13 种差异丰富的 lincRNA 可能是调节雌性山羊生殖功能的有希望的候选者。目前的结果表明，用促性腺激素反复治疗会影响激素敏感性、雌激素合成和卵巢功能。结果还表明，当对 ES 实施三种激素治疗方案时，有几种 lincRNA 可能导致对奶山羊繁殖很重要的几种基因的失调。