The hemibiotrophic fungus Zymoseptoria tritici is the causative agent of Septoria tritici leaf blotch (STB) disease of wheat (Triticum aestivum L.), the economically most damaging disease of wheat in Europe. Today, ecofriendly plant protection methods compatible with sustainable agriculture are strongly desirable. Here, we tested two chemical inducers β-aminobutyric acid (BABA) and benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) and the two biotic inducers Pseudomonas protegens CHA0 (CHA0) and P. chlororaphis PCL1391 (PCL) for their ability to induce resistance against STB in wheat seedlings. At 21 days after inoculation, only plants treated with BABA showed a smaller area covered by lesions and less pycnidia compared to the untreated control plants. We evaluated spore germination and fungal development on inoculated wheat leaves at early infection stages using calcofluor white staining. Overall, spores of Z. tritici germinated less on plants soil-drenched with BABA and BTH and their hyphal growth was significantly delayed. On the contrary, CHA0 and PCL seed treatments did not affect fungal growth in wheat leaves. In conclusion, BABA efficiently enhanced plant resistance to Z. tritici, BTH delayed fungal development at early stages while the two biotic inducers did not influence the resistance against STB disease.

所述hemibiotrophic真菌Zymoseptoria小麦是小麦的小麦壳针孢叶斑病（STB）的疾病的病原体（小麦L.），小麦的欧洲经济上最损害的疾病。如今，迫切需要与可持续农业兼容的环保植物保护方法。在这里，我们测试了两种化学诱导剂β-氨基丁酸（BABA）和苯并-（1,2,3）-噻二唑-7-碳硫代酸S-甲酯（BTH）以及两种生物诱导剂Pseudomonas protegens CHA0（CHA0）和绿腐霉PCL1391（PCL）具有诱导小麦幼苗对STB的抗性的能力。接种后第21天，与未处理的对照植物相比，仅用BABA处理的植物显示出较小的被病灶覆盖的区域和较少的脓疱病。我们使用钙荧光白染色评估了感染初期的接种小麦叶片上的孢子萌发和真菌发育。总体而言，孢子Z.小麦植物土壤湿透巴巴和BTH及其菌丝生长显著延迟发芽较少。相反，CHA0和PCL种子处理不影响小麦叶片中的真菌生长。总之，BABA有效增强了植物对Z. tritici的抗性，BTH延迟了真菌的早期发育，而两种生物诱导剂并未影响对STB疾病的抵抗力。