The introgression of genes from the wild species S. pimpinellifolium has the potential to improve tomato commercial varieties for numerous fruit quality traits. One of particular interest for fresh-market tomatoes is long shelf life (SL). Twenty-two near isogenic lines (NILs) were developed in three to four backcrosses using S. pimpinellifolium accession LA0722 as the donor parent, based on phenotypic selection for long SL and using a set of 28 SSRs for marker assisted selection. A total of 30 QTLs for fruit quality were found and 18 were subsequently validated in previous generations. A two year trial of the NILs homozygous for the LA0722 introgressions showed several novel QTLs. Fruit quality QTLs revealed an increased effect promoted by LA0722 alleles. A SL QTL was found on chromosome 9 and was consistent both years. A wild introgression on chromosome 3 showed a stable QTL for increased firmness and for yellow fruits. Therefore, the wild introgressions provide a source of variation with positive effects in fruit traits of commercial value. While obtaining new varieties, this strategy provided an effective method that integrates the QTL analysis and the prediction of positive alleles in wild germplasm.

中文翻译：

---

番茄近等基因系揭示 S. pimpinellifolium LA0722 的遗传多样性，用于果实品质和保质期育种

来自野生物种 S. pimpinellifolium 的基因渗入有可能改善番茄商业品种的众多果实品质性状。新鲜市场西红柿特别感兴趣的一项是保质期长 (SL)。基于长 SL 的表型选择和使用一组 28 个 SSR 进行标记辅助选择，使用 S. pimpinellifolium 登记号 LA0722 作为供体亲本，在三到四个回交中开发了 22 个近等基因系 (NIL)。共发现了 30 个果实品质 QTL，其中 18 个随后在前几代中得到验证。对 LA0722 基因渗入纯合 NIL 的两年试验显示了几个新的 QTL。果实品质 QTL 显示 LA0722 等位基因促进的效应增加。在第 9 号染色体上发现了一个 SL QTL，并且这两年都一致。3 号染色体上的野生基因渗入显示出稳定的 QTL，可增加硬度和黄色果实。因此，野生基因渗入提供了对具有商业价值的果实性状具有积极影响的变异来源。在获得新品种的同时，该策略提供了一种将QTL分析与野生种质阳性等位基因预测相结合的有效方法。