The present study demonstrates that the environment play a vital role in the development of tolerance in the local algal isolates towardsalpha-cypermethrin. The isolates collected from rice fields (Scenedesmus ecornis NC-M9 and Tetradesmus dimorphus NC-K2) showed significantly higher tolerance than the isolates collected from freshwater bodies far from the rice fields. Also, significant reduction in Malondialdehyde (MDA) content was noticed in S. ecornis NC-M9 after fourth and seventh day exposure to alpha-cypermethrin compared to first day exposure. However, the Superoxide Dismutase (SOD) and Peroxidase (POD) contents of S. ecornis NC-M9 enhanced at 4th and 7th day exposure to alpha-cypermethrin compared to 1st day exposure. Further, biochemical studies of all isolates show that Graesiella emersonii NC-M1(the isolate from freshwater bodies near to rice field) possesses higher contents of Chlorophyll a (Chl a), carotenoid and lipid (5.59 ± 0.36 mg·L⁻¹, 2.5 ± 0.024 mg·L⁻¹ and 28 ± 2.5% Dry Cell Weight (DCW), respectively) compared to other. This variation in biochemical parameters present in different field areas as collected and tested could be further used as potential substrates for production of bioactive compounds which have many health and environmental benefits.

Graphical abstract

本研究表明环境在当地藻类分离物中对氯氰菊酯的耐受性发展中起着至关重要的作用。从稻田收集的分离株（Scendesmus ecornis NC-M9和Tetradesmus dimorphus NC-K2）显示出比从远离稻田的淡水体收集的分离株显着更高的耐受性。同样，与第一天接触相比，在暴露于α-氯氰菊酯的第四和第七天，S。ecornis NC-M9中丙二醛（MDA）含量明显降低。但是，S。ecornis的超氧化物歧化酶（SOD）和过氧化物酶（POD）含量与第一天接触相比，在第4天和第7天接触α-氯氰菊酯的NC-M9增强。此外，所有分离物的生化研究表明，埃默森氏菌NC-M1（来自稻田附近淡水体的分离物）具有较高的叶绿素a（Chl a），类胡萝卜素和脂质含量（5.59±0.36 mg·L ^-1，分别为2.5±0.024 mg·L ^-1和28±2.5％干细胞重量（DCW）。收集和测试的在不同领域中存在的生化参数的这种变化可以进一步用作生产具有许多健康和环境益处的生物活性化合物的潜在底物。

图形概要