Polyelectrolyte multilayer coating is a promising tool to control cellular behavior. Murine C3H10T1/2 embryonic fibroblasts share many features with mesenchymal stem cells, which are good candidates for use in regenerative medicine. However, the interactions of C3H10T1/2 cells with polyelectrolyte multilayers have not been studied yet. Hence, the effect of molecular composition of biomimetic multilayers, by pairing collagen I (Col I) with either hyaluronic acid or chondroitin sulfate, based primarily on ion pairing and on additional intrinsic cross-linking was studied regarding the adhesion and differentiation of C3H10T1/2 cells. It was found that the adhesion and osteogenic differentiation of C3H10T1/2 cells were more pronounced on chondroitin sulfate-based multilayers when cultured in the absence of osteogenic supplements, which corresponded to the significant larger amounts of Col I fibrils in these multilayers. By contrast, the staining of cartilage-specific matrixes was more intensive when cells were cultured on hyaluronic acid-based multilayers. Moreover, it is of note that a limited osteogenic and chondrogenic differentiation were detected when cells were cultured in osteogenic or chondrogenic medium. Specifically, cells were largely differentiated into an adipogenic lineage when cultured in osteogenic medium or 100 ng mL⁻¹ bone morphogenic protein 2, and it was more evident on the oxidized glycosaminoglycans-based multilayers, which corresponded also to the higher stiffness of cross-linked multilayers. Overall, polyelectrolyte multilayer composition and stiffness can be used to direct cell–matrix interactions, and hence the fate of C3H10T1/2 cells. However, these cells have a higher adipogenic potential than osteogenic or chondrogenic potential.

聚电解质多层涂层是控制细胞行为的有前途的工具。小鼠C3H10T1 / 2胚胎成纤维细胞与间充质干细胞具有许多共同特征，是用于再生医学的良好候选者。然而，尚未研究C3H10T1 / 2电池与聚电解质多层的相互作用。因此，研究了仿生多层分子组成的影响，主要基于离子对和其他固有交联，通过将胶原蛋白I（Col I）与透明质酸或硫酸软骨素配对，研究了C3H10T1 / 2的粘附和分化细胞。研究发现，在不添加成骨剂的情况下培养时，在硫酸软骨素基多层膜上，C3H10T1 / 2细胞的粘附和成骨分化更为明显，这对应于这些多层中大量的Col I原纤维。相反，当在透明质酸基多层膜上培养细胞时，软骨特异性基质的染色强度更高。此外，值得注意的是，当在成骨或成软骨培养基中培养细胞时，检测到有限的成骨和成软骨分化。具体而言，当在成骨培养基或100 ng mL中培养时，细胞在很大程度上分化为成脂谱系 值得注意的是，当在成骨或成软骨培养基中培养细胞时，检测到有限的成骨和成软骨分化。具体而言，当在成骨培养基或100 ng mL中培养时，细胞在很大程度上分化为成脂谱系 值得注意的是，当在成骨或成软骨培养基中培养细胞时，检测到有限的成骨和成软骨分化。具体而言，当在成骨培养基或100 ng mL中培养时，细胞在很大程度上分化为成脂谱系^-1骨形态发生蛋白2，在氧化的基于糖胺聚糖的多层膜上更为明显，这也对应于交联多层膜的较高刚度。总体而言，聚电解质多层膜的组成和刚度可用于指导细胞与基质的相互作用，从而指导C3H10T1 / 2细胞的命运。但是，这些细胞具有比成骨或成软骨潜能高的成脂潜能。