Polyelectrolyte multilayer coating is a promising tool to control cellular behavior. Murine C3H10T1/2 embryonic fibroblasts share many features with mesenchymal stem cells, which are good candidates for use in regenerative medicine. However, the interactions of C3H10T1/2 cells with polyelectrolyte multilayers have not been studied yet. Hence, the effect of molecular composition of biomimetic multilayers, by pairing collagen I (Col I) with either hyaluronic acid or chondroitin sulfate, based primarily on ion pairing and on additional intrinsic cross-linking was studied regarding the adhesion and differentiation of C3H10T1/2 cells. It was found that the adhesion and osteogenic differentiation of C3H10T1/2 cells were more pronounced on chondroitin sulfate-based multilayers when cultured in the absence of osteogenic supplements, which corresponded to the significant larger amounts of Col I fibrils in these multilayers. By contrast, the staining of cartilage-specific matrixes was more intensive when cells were cultured on hyaluronic acid-based multilayers. Moreover, it is of note that a limited osteogenic and chondrogenic differentiation were detected when cells were cultured in osteogenic or chondrogenic medium. Specifically, cells were largely differentiated into an adipogenic lineage when cultured in osteogenic medium or 100 ng mL⁻¹ bone morphogenic protein 2, and it was more evident on the oxidized glycosaminoglycans-based multilayers, which corresponded also to the higher stiffness of cross-linked multilayers. Overall, polyelectrolyte multilayer composition and stiffness can be used to direct cell–matrix interactions, and hence the fate of C3H10T1/2 cells. However, these cells have a higher adipogenic potential than osteogenic or chondrogenic potential.

聚电解质多层涂层是控制细胞行为的有前途的工具。小鼠 C3H10T1/2 胚胎成纤维细胞与间充质干细胞具有许多共同特征，是再生医学的良好候选者。然而，C3H10T1/2 细胞与聚电解质多层的相互作用尚未研究。因此，通过将胶原蛋白 I (Col I) 与透明质酸或硫酸软骨素配对，主要基于离子配对和额外的内在交联，研究了仿生多层分子组成对 C3H10T1/2 粘附和分化的影响细胞。研究发现，在没有成骨补充剂的情况下培养时，C3H10T1/2 细胞的粘附和成骨分化在基于硫酸软骨素的多层上更加明显，这对应于这些多层中显着大量的 Col I 原纤维。相比之下，当细胞在基于透明质酸的多层膜上培养时，软骨特异性基质的染色更加强烈。此外，值得注意的是，当细胞在成骨或软骨形成培养基中培养时，检测到有限的成骨和软骨分化。具体来说，当在成骨培养基或100 ng mL ^-1骨形态发生蛋白2中培养时，细胞很大程度上分化为脂肪形成谱系，并且在基于氧化糖胺聚糖的多层上更加明显，这也对应于交联的更高刚度多层。总体而言，聚电解质多层组成和硬度可用于指导细胞-基质相互作用，从而决定 C3H10T1/2 细胞的命运。然而，这些细胞具有比成骨或软骨形成潜力更高的脂肪形成潜力。