A method for analyzing Δ⁹-tetrahydrocannabinol (THC), 11-hydroxy-Δ⁹-THC (THC-OH) and 11-nor-Δ⁹-THC-9-carboxylic acid (THC-COOH) in postmortem solid specimens using liquid chromatography–tandem mass spectrometry was developed and validated. A Stomacher instrument was used to prepare these tissues before extraction. Prior to solid phase extraction, liver, kidney, stomach, lung, brain, muscle, bladder and intestine tissues were pretreated with alkaline hydrolysis. All calibration curves were found to be linear with coefficients of determination greater than 0.99. The limit of quantification was 1.0 ng/g. Using three controls, within-run precision ranged between 1.0 and 12.0%, between-run precision ranged between 1.0 and 6.0%, and accuracy ranged between −7.0 and 8.0%. Matrix effects ranged from −21 to 24%. After matrix effects were excluded, analytical recoveries ranged from 79 to 97%. The distributions of THC, THC-OH and THC-COOH were investigated in 32 postmortem cases that tested positive for cannabinoids. This revealed new information regarding the distribution of THC metabolites in stomach, intestine and bladder. Alkaline hydrolysis was sufficient for the deglucuronidation of THC-COOH-glucuronide to its free form, THC-COOH, in all tissues of interest. In conclusion, measuring THC and its metabolites (THC-OH and THC-COOH) in tissues is crucial for any forensic toxicology detection method, especially when bodies are heavily decomposed, as solid tissues may be the only specimens available for testing.

中文翻译：

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使用LC-MS-MS对Δ9-四氢大麻酚和代谢产物进行死后组织定量的方法。

一种用于分析Δ方法⁹四氢大麻酚（THC），11-羟基Δ ⁹ -THC（THC-OH）和11-去甲Δ ⁹液相色谱-串联质谱法对死后固体样品中的-THC-9-羧酸（THC-COOH）进行了开发和验证。在提取之前，使用Stomacher仪器准备这些组织。在固相萃取之前，用碱性水解预处理肝，肾，胃，肺，脑，肌肉，膀胱和肠组织。发现所有校准曲线均为线性，测定系数大于0.99。定量限为1.0 ng / g。使用三个控件，行程内精度在1.0到12.0％之间，行程间精度在1.0到6.0％之间，精度在-7.0到8.0％之间。基质效应从-21％到24％不等。排除基质效应后，分析回收率在79％至97％之间。THC的分布，在对大麻素测试呈阳性的32例死后病例中调查了THC-OH和THC-COOH。这揭示了有关THC代谢物在胃，肠和膀胱中分布的新信息。在所有感兴趣的组织中，碱性水解足以使THC-COOH-葡糖醛酸苷去葡萄糖醛酸化为其游离形式THC-COOH。总之，测量组织中的THC及其代谢产物（THC-OH和THC-COOH）对于任何法医毒理学检测方法都是至关重要的，尤其是当身体严重分解时，因为实体组织可能是唯一可用于测试的标本。在所有感兴趣的组织中，碱性水解足以将THC-COOH-葡糖醛酸苷去葡萄糖醛酸化为其游离形式THC-COOH。总之，测量组织中的THC及其代谢产物（THC-OH和THC-COOH）对于任何法医毒理学检测方法都是至关重要的，尤其是当身体严重分解时，因为实体组织可能是唯一可用于测试的标本。在所有感兴趣的组织中，碱性水解足以使THC-COOH-葡糖醛酸苷去葡萄糖醛酸化为其游离形式THC-COOH。总之，测量组织中的THC及其代谢产物（THC-OH和THC-COOH）对于任何法医毒理学检测方法都是至关重要的，尤其是当身体严重分解时，因为实体组织可能是唯一可用于测试的标本。