This study aims to better understand the relationship between the response to acid mine drainage (AMD) stress of tolerant plants and changes in root-related bacterial communities. In this study, reed stems were planted in AMD-polluted and unpolluted soils, and high-throughput sequencing was conducted to analyze the bacterial community composition in the soil, rhizosphere, rhizoplane and endosphere. The results showed that the effect of AMD pollution on root-associated bacterial communities was greater than that of rhizo-compartments. Proteobacteria were dominant across the rhizo-compartments between treatments. The microbiomes of unpolluted treatments were enriched by Alphaproteobacteria and Betaproteobacteria and depleted in Gammaproteobacteria ranging from the rhizoplane into the endosphere. However, the opposite trend was observed in the AMD pollution treatment, namely, Gammaproteobacteria were enriched, and Alphaproteobacteria and Deltaproteobacteria were mostly depleted. In addition, endophytic microbiomes were dominated by Comamonadaceae and Rhodocyclaceae in the unpolluted treatment and by Enterobacteriaceae in the AMD-polluted soils. PICRUSt showed that functional categories associated with membrane transport, metabolism and cellular processes and signaling processes were overrepresented in the endosphere of the AMD-polluted treatment. In conclusion, our study reveals significant variation in bacterial communities colonizing rhizo-compartments in two soils, indicating that plants can recruit functional bacteria to the roots in response to AMD pollution.

中文翻译：

---

植物的根系对细菌群落的募集因酸性矿山排水污染土壤而改变。

这项研究旨在更好地理解耐性植物对酸性矿井排水（AMD）胁迫的响应与根系相关细菌群落变化之间的关系。在这项研究中，芦苇茎种植在AMD污染和未污染的土壤中，并进行了高通量测序，以分析土壤，根际，根际平面和内层土壤中的细菌群落组成。结果表明，AMD污染对根系相关细菌群落的影响大于根际区室。在处理之间的根际区隔中，细菌是显性的。无污染处理的微生物群中富含Alphaproteobacteria细菌和Betaproteobacteria细菌，而从根际平面到内层球体的Gamma proteobacteria细菌则被清除。然而，在AMD污染处理中观察到相反的趋势，即丙种细菌富集，而丙种细菌和三角洲细菌大多数被消耗。另外，在未污染的处理中，内生微生物组以Comamonadaceae和Rhodocyclaceae为主，而在AMD污染的土壤中以肠杆菌科为主。PICRUSt表明，与膜运输，代谢，细胞过程和信号传导过程相关的功能类别在被AMD污染的处理的球体内过分代表。总之，我们的研究揭示了定殖在两种土壤中的根际区室的细菌群落的显着变化，表明植物可以响应AMD污染而向根部募集功能性细菌。和α-变形杆菌和δ-变形杆菌大部分被消耗掉。另外，在未污染的处理中，内生微生物组以Comamonadaceae和Rhodocyclaceae为主，而在AMD污染的土壤中以肠杆菌科为主。PICRUSt表明，与膜运输，代谢，细胞过程和信号传导过程相关的功能类别在被AMD污染的处理的球体内过分代表。总之，我们的研究揭示了定殖在两种土壤中的根际区室的细菌群落的显着变化，表明植物可以响应AMD污染而向根部募集功能性细菌。和α-变形杆菌和δ-变形杆菌大部分被消耗掉。另外，在未污染的处理中，内生微生物组以Comamonadaceae和Rhodocyclaceae为主，而在AMD污染的土壤中以肠杆菌科为主。PICRUSt表明，与膜运输，代谢，细胞过程和信号传导过程相关的功能类别在被AMD污染的处理的球体内过分代表。总之，我们的研究揭示了定殖在两种土壤中的根际区室的细菌群落的显着变化，表明植物可以响应AMD污染而向根部募集功能性细菌。内生微生物群在未经污染的处理中以Comamonadaceae和Rhodocyclaceae为主，而在AMD污染的土壤中以肠杆菌科为主。PICRUSt表明，与膜转运，代谢，细胞过程和信号传导过程相关的功能类别在被AMD污染的处理的球体内过分代表。总之，我们的研究揭示了定殖在两种土壤中的根际区室的细菌群落的显着变化，表明植物可以响应AMD污染而向根部募集功能性细菌。内生微生物群在未经污染的处理中以Comamonadaceae和Rhodocyclaceae为主，而在AMD污染的土壤中以肠杆菌科为主。PICRUSt表明，与膜运输，代谢，细胞过程和信号传导过程相关的功能类别在被AMD污染的处理的球体内过分代表。总之，我们的研究揭示了定殖在两种土壤中的根际区室的细菌群落的显着变化，表明植物可以响应AMD污染而向根部募集功能性细菌。