Vibrio cholerae (V. cholerae) is an etiological cause of cholera implicated in several pandemics. Antibacterial activity of plant extracts has been established. However, these extracts exhibit activity at a concentration which may alter organoleptic attributes of water and food, hence limiting their application. In this light, there is need to device ways of reducing plant extracts effective levels in order to widen their application. Thus, this study was conducted to improve activities of plant ethanolic extracts through combination with other generally recognized as safe (GRAS) antimicrobials. Combination of plant extracts with sodium acetate (NaOAc) 0.4%, at pH 7.0 reduced Minimum Inhibitory Concentrations (MICs) of clove, lemon eucalyptus, rosemary and sage from 0.2% to 0.025%. At pH 6.4, combinations were more effective reducing MICs of clove, lemon eucalyptus, rosemary and sage from 0.2% to 0.0125% with NaOAc at 0.2%. At pH 7.0, the combination resulted in additive effect. Nevertheless, at pH 6.4, synergic effect was established. No interactive effect was observed with combinations involving glycine. Combination of plant extracts with NaOAc at mildly acidic pH creates a hurdle effect which may have potential application to control the growth of V. cholerae.

中文翻译：

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具有跨栏技术的植物提取物对霍乱弧菌的抗菌作用。

霍乱弧菌（霍乱弧菌）是霍乱的病因，与几场大流行有关。已经确定了植物提取物的抗菌活性。然而，这些提取物在可能改变水和食物的感官特性的浓度下表现出活性，因此限制了它们的应用。鉴于此，需要一种减少植物提取物有效水平的装置方法，以扩大其应用范围。因此，进行这项研究是为了通过与其他公认的安全（GRAS）抗菌药物联合使用来提高植物乙醇提取物的活性。在pH 7.0时，将植物提取物与0.4％的乙酸钠（NaOAc）结合使用，可使丁香，柠檬桉树，迷迭香和鼠尾草的最低抑菌浓度（MIC）从0.2％降低至0.025％。在pH值为6.4的情况下，联合使用可更有效地降低丁香，柠檬桉树，迷迭香和鼠尾草的含量从0.2％降至0.0125％，NaOAc的含量为0.2％。在pH 7.0下，该组合产生加和作用。然而，在pH 6.4下，建立了协同作用。涉及甘氨酸的组合未观察到交互作用。在中度酸性pH下，将植物提取物与NaOAc组合会产生障碍效应，这可能具有潜在的应用来控制黄瓜的生长霍乱弧菌。