ABSTRACT Strigolactone and karrikin receptors, DWARF14 (D14) and KARRIKIN INSENSITIVE 2 (KAI2), respectively, have been shown to positively regulate drought resistance in Arabidopsis thaliana by modulating abscisic acid responsiveness, anthocyanin accumulation, stomatal closure, cell membrane integrity and cuticle formation. Here, we aim to identify genes specifically or commonly regulated by D14 and KAI2 under water scarcity, using comparative analysis of the transcriptome data of the A. thaliana d14-1 and kai2-2 mutants under dehydration conditions. In comparison with wild-type, under dehydration conditions, the expression levels of genes related to photosynthesis and the metabolism of glucosinolates and trehalose were significantly changed in both d14-1 and kai2-2 mutant plants, whereas the transcript levels of genes related to the metabolism of cytokinins and brassinosteroids were significantly altered in the d14-1 mutant plants only. These results suggest that cytokinin and brassinosteroid metabolism might be specifically regulated by the D14 pathway, whereas photosynthesis and metabolism of glucosinolates and trehalose are potentially regulated by both D14 and KAI2 pathways in plant response to water scarcity.

中文翻译：

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独脚金内酯和karrikin信号调控拟南芥对缺水胁迫抗性的不同策略

摘要 独脚金内酯和 karrikin 受体 DWARF14 (D14) 和 KARRIKIN INSENSITIVE 2 (KAI2) 分别通过调节脱落酸反应性、花青素积累、气孔闭合、细胞膜完整性和角质层形成，已被证明可正向调节拟南芥的抗旱性。在这里，我们的目标是在缺水条件下通过对拟南芥 d14-1 和 kai2-2 突变体在脱水条件下的转录组数据进行比较分析，来鉴定缺水条件下受 D14 和 KAI2 特异性或普遍调控的基因。与野生型相比，在脱水条件下，d14-1和kai2-2突变植株的光合作用及硫代葡萄糖苷和海藻糖代谢相关基因的表达水平发生显着变化，而与细胞分裂素和油菜素类固醇代谢相关的基因转录水平仅在 d14-1 突变植物中显着改变。这些结果表明，细胞分裂素和油菜素类固醇的代谢可能受 D14 途径的特异性调节，而硫代葡萄糖苷和海藻糖的光合作用和代谢可能受 D14 和 KAI2 途径的调节，以应对植物对缺水的反应。