ABSTRACT

Brevicoryne brassicae is one of the most important pests of wide range of cruciferous host plants. In this study, the inhibitory effects of aqueous extracts of five cultivars of common bean (Phaseolus vulgaris L.), that includes white beans, pinto, red, cowpea and keshavarzi on the activity of the α-amylase enzyme of B. brassicae was studied. Aphids were reared under laboratory conditions with 25 ± 1°C, 60 ± 5% RH and 16 L:8D h photoperiods. According to obtained results, the cytotoxic effect of extracts of the highest concentration of 0.05 mg ml⁻¹ and within 72 hours had the best performance and mortality. Also, all bean extracts had an inhibitory effect on insect α-amylase enzyme and red bean had the highest inhibitory effect (IC₅₀ = 0.223). The effects of the bean inhibitor on α-amylase of B. brassicae showed a dose-dependent manner of inhibition, e.g., less inhibition of enzyme activity (around 10%) with a lower dose (0.25 mg protein) and high inhibition of enzyme activity (around 90%) when a high dose of inhibitor was used (1 mg protein). Based on the data presented in this study, it could be said that the bean extract has good inhibitory activity on B. brassicae α-amylase.

摘要

Brevicoryne brasicae是十字花科寄主植物最重要的害虫之一。在这项研究中，菜豆（的5个品种的含水萃取的抑制作用菜豆L.），包括白扁豆，斑，红色，豇豆和keshavarzi上的α淀粉酶的活性B.甘蓝进行了研究. 蚜虫在实验室条件下饲养，温度为 25 ± 1°C、60 ± 5% RH 和 16 L:8D h 光周期。根据所得结果，最高浓度为0.05 mg ml ^-1且在72小时内的提取物的细胞毒作用具有最好的性能和死亡率。此外，所有豆类提取物对昆虫 α-淀粉酶都有抑制作用，红豆的抑制作用最高（IC ₅₀ = 0.223）。豆类抑制剂对芸苔属植物α-淀粉酶的影响显示出剂量依赖性的抑制方式，例如较低剂量（0.25mg蛋白质）对酶活性的抑制较小（约10％）和对酶活性的高度抑制（约 90%）当使用高剂量抑制剂（1 毫克蛋白质）时。根据本研究中提供的数据，可以说豆提取物对甘蓝芽孢杆菌α-淀粉酶具有良好的抑制活性。