We studied the bioactive compound of the fungus Simplicillium chinense strain Snef5, isolated from its culture filtrate. Following purification by chromatography, the structure of the secondary metabolite, identified as 2,6-pyridinedicarboxylic acid, was determined by spectroscopic methods (IR, ¹H, and ¹³C NMR) and scanning electron microscopy (SEM). The metabolite exhibited antimicrobial activity against M. incognita with an LD50 of 0.229 mg/ml. The light microscope was used to observe the surface and internal cellular micro-structure of the nematodes. The results of the microscopy indicated that the anatomy of the J₂ of M. incognita was disrupted following exposure to 2,6-pyridinedicarboxylic acid. This is the first report on the activity of 2,6-pyridinedicarboxylic acid of S. chinense metabolites on the root knot nematode. It showed a high level of nematicidal activity. Our data on the inhibitory effects of 2,6-pyridinedicarboxylic acid suggest that secondary metabolites from Snef5 have potential use as novel biological control agents.

我们研究了真菌的生物活性化合物Simplicillium楸应变Snef5，从其培养物滤液中分离。在通过色谱法纯化之后，通过光谱方法（IR，¹ H和¹³ C NMR）和扫描电子显微镜（SEM）确定了鉴定为2，6-吡啶二羧酸的次级代谢物的结构。代谢物表现出对抗菌活性南方根结线虫用0.229毫克/毫升的LD 50。用光学显微镜观察线虫的表面和内部细胞的微观结构。该显微镜的结果表明，j的解剖结构₂的南方根结线虫暴露于2，6-吡啶二羧酸后，其被破坏。这是关于根结线虫的中华链球菌代谢物的2，6-吡啶二羧酸活性的首次报道。它显示出高水平的杀线虫活性。我们对2，6-吡啶二羧酸抑制作用的数据表明，Snef5的次生代谢产物有可能用作新型生物防治剂。