Mortalities of bivalve larvae and spat linked with Vibrio spp. infection have been described in hatcheries since 1959, causing potential development of resistant bacteria. A reliable and sustainable solution to this problem is yet to be developed. Potential treatment of bacterial infection with bacteriophages is gaining interest in aquaculture as a more sustainable option for managing Vibrio spp. infection. This study assessed the effectiveness of bacteriophages (Φ-5, Φ-6, and Φ-7) against pathogenic Vibrio isolates (USC-26004 and USC-26005). These phage isolates were found to belong to the Myoviridae viral family. A total of 212 ORFs of Φ-5 were identified and annotated. The genome of this phage contained putative thymidine kinase and lysin enzyme. During infections with phages, the OD values of the isolates USC-26005 and USC-26004 remained stable at a much lower reading compared to the control after 9 h of incubation. Mortality rate of oyster (Saccostrea glomerata) larvae was 28.2 ± 3.5% in the bacteriophage treatment group, compared to 77.9 ± 9.1% in the bacterial treatment group after 24 h incubation. Findings of this study indicate that lytic phages might be utilized as potential bio-control agents of luminescent bacterial disease in oyster hatcheries.

中文翻译：

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噬菌体在控制牡蛎（Saccostrea glomerata）幼虫中溶藻弧菌污染中的应用。

双壳虫幼虫和幼虫的死亡率与弧菌属菌种有关。自1959年以来，孵化场就已经描述了这种细菌的感染，从而引起耐药菌的潜在发展。尚未开发出解决该问题的可靠且可持续的解决方案。作为一种更可持续的管理弧菌属物种的选择，用噬菌体对细菌感染的潜在治疗正在引起人们的关注。感染。这项研究评估了噬菌体（Φ-5，Φ-6和Φ-7）针对病原性弧菌分离株（USC-26004和USC-26005）的有效性。发现这些噬菌体分离株属于肌病毒科病毒家庭。总共鉴定并注释了212个Φ-5的ORF。该噬菌体的基因组包含推定的胸苷激酶和溶素酶。孵育9小时后，在噬菌体感染期间，与对照相比，分离株USC-26005和USC-26004的OD值保持稳定，读数低得多。噬菌体处理组中的牡蛎（Saccostrea glomerata）幼虫的死亡率为28.2±3.5％，而24小时孵育后细菌处理组的死亡率为77.9±9.1％。这项研究的发现表明，溶菌噬菌体可能被用作牡蛎孵化场中潜在的发光细菌疾病生物防治剂。