Study of the efficacy of Aureobasidium strains belonging to three different species: A. pullulans, A. subglaciale and A. melanogenum against Botrytis cinerea of tomato,Annals of Applied Biology

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Study of the efficacy of Aureobasidium strains belonging to three different species: A. pullulans, A. subglaciale and A. melanogenum against Botrytis cinerea of tomato
Annals of Applied Biology ( IF 2.2 ) Pub Date : 2020-08-03 , DOI: 10.1111/aab.12627
Alessandra Di Francesco _{1,

2} , Michele Di Foggia ₃ , Janja Zajc ₄ , Nina Gunde‐Cimerman ₅ , Elena Baraldi _{1,

2}

Affiliation

The difference in antagonistic activity against the causal agent of grey mould (Botrytis cinerea) of tomato between Aureobasidium strains belonging to three different species, namely A. pullulans, A. melanogenum and A. subglaciale, was evaluated by in vitro and in vivo assays. In the yeast–pathogen direct interaction experiment, all the strains significantly reduced B. cinerea growth, with A. melanogenum the least efficient species (17.8% of reduction) compared to A. pullulans and subglaciale (22 and 27.8%). The non‐volatile metabolites produced by all three species reduced mycelial growth between 95 and 100%. These metabolites were characterised by FT‐IR spectroscopy as polysaccharides, lytic enzymes, siderophores and antibiotics. The inhibitory effect of Aureobasidium strains on pathogenic enzymes such as xylanase, polygalacturonase and pectinase was measured showing A. pullulans strains as capable of strong inhibition of xylanase, an enzyme directly related to the virulence of necrotrophic pathogens such as B. cinerea. Our data demonstrate that the different species of Aureobasidium isolated from a range of non‐conventional environments exerted variable efficacy against B. cinerea, with A. pullulans as the most active species followed by A. subglaciale and A. melanogenum as ineffective and not suitable for biocontrol applications.

中文翻译：

属于三种不同物种的金黄色葡萄球菌菌株对番茄番茄灰霉病的功效研究：A。Pullulans，A。subglaciale和Alanogenogenum

通过体外和体内试验评估了属于三个不同种类的金黄色葡萄球菌菌株（即A.pululans，A。melanogenum和A. subglaciale）之间对番茄灰霉病（灰葡萄孢）的致病因子的拮抗活性的差异。在酵母-病原体直接相互作用实验中，所有菌株均显着降低了灰葡萄芽孢杆菌的生长，而黑曲霉相比于支链霉菌和冰gla亚目，其效率最低（减少了17.8％）。（22和27.8％）。这三种物种产生的非挥发性代谢物均使菌丝体生长降低了95％至100％。这些代谢产物通过FT-IR光谱表征为多糖，裂解酶，铁载体和抗生素。的抑制作用短梗霉的菌株对致病酶如测定表示木聚糖酶，多聚半乳糖和果胶酶A.霉菌株作为能够木聚糖酶的强抑制作用，酶直接相关的死体营养病原体如致病灰霉病。我们的数据表明，从一系列非常规环境中分离出的不同种类的金黄色葡萄球菌对灰葡萄孢具有不同的功效，支链球菌是最活跃的物种，其次是冰下曲霉和黑色素曲霉，它们无效且不适合生物防治应用。

更新日期：2020-08-03

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