The ability to sense proteins and protein‐related interactions at the single‐molecule level is becoming of increasing importance to understand biological processes and diseases better. Single‐molecule sensors, such as nanopores have shown substantial promise for the label‐free detection of proteins; however, challenges remain due to the lack of selectivity and the need for relatively high analyte concentrations. An aptamer‐functionalized nanopore extended field‐effect transistor (nexFET) sensor is reported here, where protein transport can be controlled via the gate voltage that in turn improves single‐molecule sensitivity and analyte capture rates. Importantly, these sensors allow for selective detection, based on the choice of aptamer chemistry, and can provide a valuable addition to the existing methods for the analysis of proteins and biomarkers in biological fluids.

中文翻译：

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使用适体功能化的纳米孔扩展场效应晶体管对蛋白质的选择性传感

为了更好地了解生物过程和疾病，在单分子水平上感知蛋白质和蛋白质相关相互作用的能力变得越来越重要。单分子传感器，例如纳米孔，已显示出无标记检测蛋白质的巨大希望；但是，由于缺乏选择性以及需要相对较高的分析物浓度，挑战依然存在。此处报道了适体功能化的纳米孔扩展场效应晶体管（nexFET）传感器，可通过栅极电压控制蛋白质的运输，从而提高单分子灵敏度和分析物捕获率。重要的是，这些传感器可以根据适体化学成分的选择进行选择性检测，