Meat and bone meal (MBM) is an important protein source used in animal feeds. However, as the composition and availability of amino acids (AAs) in MBM fluctuate markedly, it is important to verify the magnitude of these parameters in different MBMs. In this study, the AA compositions of 19 MBMs were analyzed to confirm variations in lysine (Lys), threonine (Thr), and tryptophan (Trp), then which were compared with those of soybean meal (SBM) and fish meal (FM). Instability in Lys, Thr, and Trp availabilities in six MBMs were also considered after estimation using the slope‐ratio growth assay technique in rats. Variations in AA composition were evaluated using the coefficient of variance (CV: Standard deviation/Mean). CVs for Lys, Thr, and Trp content were 9.40, 11.83, and 18.12 in MBM, 2.71, 2.48, and 3.19 in SBM, and 10.09, 10.44, and 13.47 in FM. Furthermore, means and SDs for Lys, Thr, and Trp availabilities in MBM were 53.3 ± 10.4% (CV: 19.5), 65.9 ± 17.6% (CV: 26.6), and 83.2 ± 11.2% (CV: 13.5), respectively. These results provide the first evidence that variations in MBM AA compositions were 3.5 to 5.7 times higher than those in SBM, but similar to FM, and that the large variation in availability substantially existed.

中文翻译：

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肉和骨粉中赖氨酸、苏氨酸和色氨酸可用性的变化，通过生长大鼠的斜率比生长测定技术估计。

肉骨粉 (MBM) 是动物饲料中使用的重要蛋白质来源。然而，由于 MBM 中氨基酸 (AA) 的组成和可用性显着波动，因此验证不同 MBM 中这些参数的大小非常重要。在这项研究中，分析了 19 种肉骨粉的 AA 组成，以确认赖氨酸 (Lys)、苏氨酸 (Thr) 和色氨酸 (Trp) 的变化，然后将其与豆粕 (SBM) 和鱼粉 (FM) 的变化进行比较. 在使用大鼠的斜率比生长测定技术进行估计后，还考虑了六种 MBM 中 Lys、Thr 和 Trp 可用性的不稳定性。使用方差系数（CV：标准差/平均值）评估 AA 组成的变化。Lys、Thr 和 Trp 含量的 CV 在 MBM 中为 9.40、11.83 和 18.12，在 SBM 中为 2.71、2.48 和 3.19，在 FM 中为 10.09、10.44 和 13.47。此外，MBM 中 Lys、Thr 和 Trp 可用性的平均值和 SD 分别为 53.3 ± 10.4% (CV：19.5)、65.9 ± 17.6% (CV：26.6) 和 83.2 ± 11.2% (CV：13.5)。这些结果提供了第一个证据，表明 MBM AA 成分的变化比 SBM 高 3.5 至 5.7 倍，但与 FM 相似，并且可用性的大变化实质上存在。