Screening soybean cyst nematode effectors for their ability to suppress plant immunity.,Molecular Plant Pathology

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Screening soybean cyst nematode effectors for their ability to suppress plant immunity.
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2020-07-16 , DOI: 10.1111/mpp.12972
Gennady Pogorelko ₁ , Jianying Wang ₂ , Parijat S Juvale ₁ , Melissa G Mitchum _{2,

3} , Thomas J Baum ₁

Affiliation

The soybean cyst nematode (SCN), Heterodera glycines, is one of the most destructive pathogens of soybeans. SCN is an obligate and sedentary parasite that transforms host plant root cells into an elaborate permanent feeding site, a syncytium. Formation and maintenance of a viable syncytium is an absolute requirement for nematode growth and reproduction. In turn, sensing pathogen attack, plants activate defence responses and may trigger programmed cell death at the sites of infection. For successful parasitism, H. glycines must suppress these host defence responses to establish and maintain viable syncytia. Similar to other pathogens, H. glycines engages in these molecular interactions with its host via effector proteins. The goal of this study was to conduct a comprehensive screen to identify H. glycines effectors that interfere with plant immune responses. We used Nicotiana benthamiana plants infected by Pseudomonas syringae and Pseudomonas fluorescens strains. Using these pathosystems, we screened 51 H. glycines effectors to identify candidates that could inhibit effector‐triggered immunity (ETI) and/or pathogen‐associated molecular pattern (PAMP)‐triggered immunity (PTI). We identified three effectors as ETI suppressors and seven effectors as PTI suppressors. We also assessed expression modulation of plant immune marker genes as a function of these suppressors.

中文翻译：

筛选大豆胞囊线虫效应子抑制植物免疫的能力。

大豆胞囊线虫（SCN），大豆胞囊线虫，是大豆最具破坏性的病原体之一。 SCN 是一种专性、静止的寄生虫，它将宿主植物根细胞转化为一个精心设计的永久摄食位点，即合胞体。可行的合胞体的形成和维持是线虫生长和繁殖的绝对要求。反过来，在感知病原体攻击时，植物会激活防御反应，并可能在感染部位引发程序性细胞死亡。为了成功寄生，甘氨酸必须抑制这些宿主防御反应以建立和维持可行的合胞体。与其他病原体类似，甘氨酸嗜血杆菌通过效应蛋白与其宿主进行这些分子相互作用。本研究的目的是进行全面筛选，以确定干扰植物免疫反应的甘氨酸异丙酸效应子。我们使用被丁香假单胞菌和荧光假单胞菌菌株感染的本塞姆氏烟草植物。利用这些病理系统，我们筛选了 51 个甘氨酸嗜血杆菌效应子，以确定可以抑制效应子触发免疫 (ETI) 和/或病原体相关分子模式 (PAMP) 触发免疫 (PTI) 的候选者。我们确定了三个效应器作为 ETI 抑制器，七个效应器作为 PTI 抑制器。我们还评估了植物免疫标记基因的表达调节作为这些抑制子的功能。

更新日期：2020-07-16

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