Sensitive protein detection and visualization using protein-binding peptides.,Microscopy Research and Technique

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Sensitive protein detection and visualization using protein-binding peptides.
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2020-07-16 , DOI: 10.1002/jemt.23482
Lu Ma ₁ , Tao Yang ₁ , Chuanbin Mao ₂ , Mingying Yang ₃

Affiliation

Silk sericin (SS) as a silk protein has important applications in biomedical and textile fields, and thus finding a facile method for detecting and imaging it is very important. Here, we show that a free SS‐binding peptide, labeled with horseradish peroxidase and fluorescein isothiocyanate, could be used as a probe to determine the concentration of SS (from 1 to 100 μg/ml) in a dilute solution with a detection limit of 0.073 μg/ml and visualize the distribution of SS on a substrate under a fluorescent microscope, respectively. This work highlights the importance in discovering a protein‐binding peptide and the power of such a peptide for the detection and imaging of technologically important proteins.

中文翻译：

使用蛋白质结合肽进行灵敏的蛋白质检测和可视化。

丝胶蛋白（SS）作为一种丝蛋白在生物医学和纺织领域有着重要的应用，因此寻找一种简便的检测和成像方法非常重要。在这里，我们展示了用辣根过氧化物酶和异硫氰酸荧光素标记的游离 SS 结合肽可用作探针来确定稀释溶液中 SS 的浓度（从 1 到 100 μg/ml），检测限为0.073 μg/ml 并分别在荧光显微镜下观察 SS 在基材上的分布。这项工作强调了发现蛋白质结合肽的重要性以及这种肽在检测和成像技术上重要的蛋白质方面的能力。

更新日期：2020-09-09

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