Abstract In this study, we have designed developed the microbubbles (MBs) coated Cu-Se nanoparticles (MBs@Cu-Se NPs) was efficiently synthesized and combined with the Ultrasound for potential anticancer effects in ovarian carcinoma cells. The fabricated nanoparticles are perceived as the well-known spherical size by SEM and TEM analysis. The hydrodynamic parameters of the MBs@Cu-Se NPs confirm via DLA analysis. Further, in vitro cancer potential of the Cu-Se, MBs@Cu-Se NPs and MBs@Cu-Se NPs with Ultrasound efficiently kills the ovarian cancer cells (A2780 and CisRA2780) and reduced the cell viability in a dose-dependent manner. The MBs@Cu-Se NPs with Ultrasound displayed remarkably higher amounts of apoptosis of the ovarian cancers, which was confirmed by the various biochemical assays (AO-EB), and Hoechst-33,258 by nuclear staining. Additionally, we examined the apoptosis mechanism through the flow cytometry technique. The results reveal that the MBs@Cu-Se NPs with Ultrasound significantly induce apoptosis in both cancer cells. These results suggest that the in vitro cytotoxicity potential of MBs@Cu-Se NPs with Ultrasound combination therapy against ovarian carcinoma. Overall, new approaches of combination therapy of MBs@Cu-Se NPs with Ultrasound could be a promising alternative strategy and efficient chemotherapy as well as radiotherapy.

中文翻译：

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脂质微泡包覆铜和硒纳米粒子的工程：超声波刺激辐射对人类卵巢癌细胞的抗癌活性

摘要 在本研究中，我们设计了有效合成微泡 (MBs) 包覆的 Cu-Se 纳米粒子 (MBs@Cu-Se NPs) 并结合超声对卵巢癌细胞具有潜在的抗癌作用。通过 SEM 和 TEM 分析，制造的纳米颗粒被认为是众所周知的球形尺寸。MBs@Cu-Se NPs 的流体动力学参数通过 DLA 分析得到证实。此外，Cu-Se、MBs@Cu-Se NPs 和 MBs@Cu-Se NPs 与超声的体外致癌潜力可有效杀死卵巢癌细胞（A2780 和 CisRA2780）并以剂量依赖性方式降低细胞活力。带有超声的 MBs@Cu-Se NPs 显示出显着更高的卵巢癌凋亡量，这通过各种生化测定 (AO-EB) 和 Hoechst-33,258 的核染色证实。此外，我们通过流式细胞术技术检查了细胞凋亡机制。结果表明，MBs@Cu-Se NPs 与超声显着诱导两种癌细胞的凋亡。这些结果表明 MBs@Cu-Se NPs 与超声联合治疗卵巢癌的体外细胞毒性潜力。总体而言，MBs@Cu-Se NPs 与超声联合治疗的新方法可能是一种有前景的替代策略和有效的化疗和放疗。