The presence of Toxoplasma gondii in zoos is cause of alert because many susceptible species kept in captivity die of clinical toxoplasmosis. Moreover, excretion of T. gondii oocysts by infected captive wild felines into the facilities could pose a risk to workers. Herbivores in wild collections can serve as sentinels of local transmission, since they get infected by the consumption of oocysts present in ground or water. Both herbivores and felids may reveal the parasite variants which are circulating in the region. We determined the seroprevalence of T. gondii in European mouflons (n = 55) and wild felines (n = 15) from a private zoological collection located in the Eastern region of México, as well as the incidence in 41 of the mouflons using ELISA. The prevalence of T. gondii in mouflons was 14.5% (n = 55) and 17.1% (n = 41) in 2011 and 19.5% in 2012. The estimated incidence was 9.8%–12.2%. In wild felines the frequency was 80%. Four sero-positive animals (two mouflons and the two oldest African lions) were euthanized. Histopathology, conventional PCR (for B1 and SeqRep529 loci) and molecular characterization were carried out. All euthanized animals were positive to T. gondii by PCR. We identified a triple infection (I + II + III) in the brain of a mouflon. In conclusion, a high infective pressure of T. gondii in the collection was found, supported by changes in its prevalence in European mouflons. A high prevalence of infection in wild felines was determined. At least four genotypes of T. gondii are present in herbivores and carnivores, and one mouflon had a mixed infection.

动物园中存在弓形虫是引起警觉的原因，因为圈养的许多易感物种死于临床弓形虫病。此外，被感染的圈养野生猫科动物将弓形虫卵囊排泄到设施中可能对工人构成危险。野生收集物中的食草动物可以被当地传播的定点服务，因为它们被地面或水中卵囊的消耗所感染。食草动物和猫科动物都可能发现该区域中正在传播的寄生虫变种。我们确定了弓形虫的血清阳性率来自墨西哥东部地区的私人动物学收藏品中的欧洲mo足动物（n = 55）和野生猫科动物（n = 15），以及使用ELISA的41中41足动物的发病率。2011年和2012年，弓形虫在Mouflon中的患病率为14.5％（n = 55）和17.1％（n = 41），在2012年为19.5％。估计发病率为9.8％–12.2％。在野生猫科动物中，频率为80％。安乐死了四只血清阳性动物（两只穆福隆和两只最古老的非洲狮子）。进行了组织病理学，常规PCR（用于B1和SeqRep529基因座）和分子表征。通过PCR，所有安乐死的动物对弓形虫均呈阳性。我们在mouflon的大脑中发现了三次感染（I + II + III）。总之，传染病的压力很大在该系列中发现了弓形虫（T. gondii），并得到了欧洲mouflons中其流行程度的变化的支持。确定了野生猫科动物的高感染率。食草动物和食肉动物中至少存在四种弓形虫基因型，并且一个mo足动物混合感染。