Abstract This work succeeded in the preparation of a nano-biocomposite material based on the use of poly-3-hydroxybutyrate-co-hydroxyhexanoate nanoparticles (PHBHHx-NPs) within a scaffold of whey protein (WP) based films. The experimental conditions for PHBHHx-NPs preparation by solvent-evaporation technique were set up, and the obtained NPs characterized. Dynamic light scattering analyses showed that PHBHHx-NPs are stable, exhibiting a zeta-potential value close to −40 mV and a Z-average size of 80 nm. Morphological characterization by transmission and scanning electron microscopy confirmed nanoparticle average dimensions. The addition of PHBHHx-NPs to WP-based films improved the mechanical properties of the derived bioplastics, producing more extensible materials preserving their mechanical resistance. The grafting of PHBHHx-NPs as material fillers also enhanced the film barrier properties towards O2, the permeability to both water vapor and CO2 remaining unaffected.

中文翻译：

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聚（3-羟基丁酸酯-共羟基己酸酯）纳米颗粒的设计和表征及其在基于乳清蛋白的纳米复合材料中的接枝

摘要 这项工作基于在基于乳清蛋白 (WP) 的薄膜支架内使用聚 3-羟基丁酸酯-共羟基己酸酯纳米颗粒 (PHBHHx-NPs) 成功制备了纳米生物复合材料。建立了溶剂蒸发法制备PHBHHx-NPs的实验条件，并对所得NPs进行了表征。动态光散射分析表明 PHBHHx-NPs 是稳定的，zeta 电位值接近 -40 mV，Z 平均尺寸为 80 nm。通过透射和扫描电子显微镜的形态表征证实了纳米颗粒的平均尺寸。将 PHBHHx-NP 添加到基于 WP 的薄膜中改善了衍生生物塑料的机械性能，生产出更具延展性的材料，同时保持其机械阻力。