Abstract

The halophyte Kalidium foliatum (Pall.) Moq. exhibits strong tolerance to salinity. Salt stress leads to reactive oxygen species (ROS) production, and high contents of ROS are harmful to plants. The phospholipid hydroperoxide glutathione peroxidase (PHGPx) responds positively to damage induced by ROS. To understand the mechanism of tolerance to salt stress in halophytes, a PHGPx gene of K. foliatum was cloned, and its expression pattern was analysed under salt treatment. Treatments of whole plants were carried out with 0, 150, 200, 250, 300, 350 or 400 mM sodium chloride for 72 h. The extraction of total RNA from 4‑month-old seedlings was conducted using the leaves of both treated and untreated plants. The full-length cDNA of KfPHGPx was cloned with reverse transcription PCR (RT-PCR), the KfPHGPx expression patterns were analysed using semi-quantitative RT-PCR and real-time PCR, and the activities of phospholipid hydroperoxide glutathione peroxidase enzyme in K. foliatum were examined. The full-length cDNA clone encoding the full-length sequence consisted of 714 bp of nucleotides and included an open reading frame (ORF) that encoded a polypeptide of 237 amino acids. The deduced amino acid sequence of the KfPHGPx gene has significant similarity with Spinacia oleracea (XP_021837527.1) and Sesuvium portulacastrum (ADE07246.1). The semi-quantitative RT-PCR and real-time PCR analyse showed that KfPHGPx expression can be markedly induced by moderate salt treatment.

中文翻译：

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NaCl处理下叶酸钾中磷脂氢过氧化物谷胱甘肽过氧化物酶的分子克隆及表达模式。

摘要

盐生植物叶形钾（Pall。）对盐分具有很强的耐受性。盐胁迫导致活性氧（ROS）的产生，并且高含量的ROS对植物有害。磷脂氢过氧化物谷胱甘肽过氧化物酶（PHGPx）对ROS诱导的损伤有积极的反应。要理解的耐受性在盐生植物盐胁迫的机构，PHGPx的基因K.爪爪被克隆，并在盐处理分析其表达模式。整个植物用0、150、200、250、300、350或400 mM氯化钠处理72小时。使用处理过的植物和未处理过的植物的叶子从4个月大的幼苗中提取总RNA。KfPHGPx的全长cDNA用逆转录PCR（RT-PCR）克隆，所述KfPHGPx表达模式，使用半定量RT-PCR和实时PCR分析，与磷脂氢谷胱甘肽过氧化物酶的酶的活性K.爪爪进行了研究。编码全长序列的全长cDNA克隆由714 bp的核苷酸组成，并包括一个开放阅读框（ORF），其编码237个氨基酸的多肽。推导的KfPHGPx基因的氨基酸序列与菠菜（XP_021837527.1）和东南葡萄（Susuvium portulacastrum）（ADE07246.1）具有显着相似性。半定量RT-PCR和实时PCR分析表明，KfPHGPx 适量的盐处理可以明显诱导其表达。