Abstract

The expression of the TCP transcription factor family genes depends on miRNA, whose effect on the translation of their mRNA is poorly studied. Interactions between miRNA and mRNA were studied with the MirTarget program, which allows estimating of quantitative characteristics of binding of the whole nucleotide sequence of miRNA to mRNA. The analysis of binding of 125 miRNAs to mRNAs of 28 genes of the TCP family of Triticum aestivum L. revealed eight target genes for miR319-3p, miR444a-3p, miR5086-5p, miR9666a-3p, and miR9780-3p. miRNA binding sites in mRNA of the TCP family genes of T. aestivum were located in the CDS only. Only 12 of 22 mRNAs of the ТСР family genes of Oryza sativa L. bounded to the miR1437b-5p, miR1846a-5p, miR1848-5p, miR1858a-5p, miR1858b-5p, miR1861d-5p, miR1861h-5p, miR2102-3p, miR2102-5p, miR2919, miR2925-5p, miR319a-3, miR408-3p, miR5075-3p, and miR5819-5p out of 738 miRNAs in total. miRNA binding sites were located in the 5'UTR and CDS regions of mRNAs of the TCP family genes of O. sativa. Only 10 of 46 TCP family genes of Zea mays L. were shown to be targets for the miR164g-3p, miR164h-5p, miR166a-5p, miR168a-5p, miR171d-5p, miR399d-3p, and miR408a-3p out of 325 miRNAs of Z. mays. The zma-miR166a-5p was bound to mRNAs of the genes GRMZM2G031905_P01, GRMZM2G055024_P01, GRMZM2G062711_P01, and GRMZM2G170232_P01. Two miRNAs were shown to bind to mRNAs of the genes AC213524.3_FGP003, AC233950.1_FGP002, and GRMZM2G034638_P01. Only one miRNA was bound to mRNAs of the genes GRMZM2G035944_P01, AC190734.2_FGP003, and AC205574.3_FGP006. miRNA binding sites were located in the 5'UTR and CDS regions of mRNAs of the TCP family genes of Z. mays. Ten of 27 TCP family genes of Arabidopsis thaliana (L.) Heynh. were shown to be targets for the miR319-3р, miR4228-5p, miR4228-3p, miR5021-5p, miR5658-5p, and miR8181-5p out of 429 miRNAs of A. thaliana. mRNAs of the AT1G53230 and AT2G31070 genes had binding sites for the miR319c-3p, miR5021-5p, and miR5658-5p, while mRNA of the AT3G15030 gene had binding site for the miR319a-3p and miR4228-3p. Two miRNAs bounded to mRNAs of the genes AT1G69690, AT3G02150, and AT3G47620. One miRNA bounded to mRNAs of the genes AT1G30210, AT4G18390, and AT5G08330. miRNA binding sites were located in the 5'UTR, CDS and 3'UTR regions of mRNA of the TCP gene family of A. thaliana. mRNAs of six groups of the TCP family genes had binding sites for the miR319-3p, miR444a-3p, miR5021-5p, miR5658-5p, and miR2102-5p, which encode the oligopeptides QRGPLQS, STSETS, SSSSSS, HHHHHH, GGGGGG, and AAAAAA conservative in the TCP family proteins of different plant species. Quantitative characteristics of miRNA binding to mRNAs of plant transcription factors of the TCP family, which participate in regulation of growth and development of plants, have been predicted.

中文翻译：

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预测植物TCP转录因子基因的mRNA中miRNA潜在结合位点的特征

摘要

TCP转录因子家族基因的表达依赖于miRNA，而miRNA对其mRNA翻译的影响研究很少。使用MirTarget程序研究了miRNA与mRNA之间的相互作用，该程序可以估算miRNA整个核苷酸序列与mRNA结合的定量特征。分析125个miRNA与普通小麦的TCP家族的28个基因的mRNA的结合，发现miR319-3p，miR444a-3p，miR5086-5p，miR9666a-3p和miR9780-3p的八个靶基因。小麦锥虫TCP家族基因的mRNA中的miRNA结合位点仅位于CDS中。水稻ТСР家族基因的22个mRNA中只有12个L.与miR1437b-5p，miR1846a-5p，miR1848-5p，miR1858a-5p，miR1858b-5p，miR1861d-5p，miR1861h-5p，miR2102-3p，miR2102-5p，miR2919，miR2925-5p，miR319a-3绑定，总共738个miRNA中的miR408-3p，miR5075-3p和miR5819-5p。miRNA结合位点位于水稻TCP家族基因mRNA的5'UTR和CDS区。在325个中，仅显示了Zea mays L.的46个TCP家族基因中的10个是miR164g-3p，miR164h-5p，miR166a-5p，miR168a-5p，miR171d-5p，miR399d-3p和miR408a-3p的靶标玉米Z的miRNA。zma-miR166a-5p与基因GRMZM2G031905_P01，GRMZM2G055024_P01，GRMZM2G062711_P01和GRMZM2G170232_P01的mRNA结合。显示了两个miRNA与AC213524.3_FGP003，AC233950.1_FGP002和GRMZM2G034638_P01基因的mRNA结合。只有一个miRNA与GRMZM2G035944_P01，AC190734.2_FGP003和AC205574.3_FGP006基因的mRNA结合。miRNA的结合位点位于玉米Z TCP家族基因mRNA的5'UTR和CDS区。拟南芥（L.）Heynh的27个TCP家族基因中的10个。被证明是拟南芥中429个miRNA中miR319-3р，miR4228-5p，miR4228-3p，miR5021-5p，miR5658-5p和miR8181-5p的靶标。AT1G53230和AT2G31070基因的mRNA具有miR319c-3p，miR5021-5p和miR5658-5p的结合位点，而AT3G15030基因的mRNA具有miR319a-3p和miR4228-3p的结合位点。两个miRNA与AT1G69690，AT3G02150和AT3G47620基因的mRNA结合。一个miRNA与AT1G30210，AT4G18390和AT5G08330基因的mRNA结合。miRNA结合位点位于拟南芥TCP基因家族mRNA的5'UTR，CDS和3'UTR区。。六组TCP家族基因的mRNA具有miR319-3p，miR444a-3p，miR5021-5p，miR5658-5p和miR2102-5p的结合位点，它们编码寡肽QRGPLQS，STSETS，SSSSSS，HHHHHH，GGGGGG和AAAAAA位于不同植物物种的TCP家族蛋白中。已经预测了miRNA与TCP家族的植物转录因子的mRNA结合的定量特征，其参与调节植物的生长和发育。