Low temperature (LT) and Ascochyta blight are two major stresses in chickpea (Cicer arietinum L.) cultivation. After exposure to LT treatments (acclimation, LT, acclimation + LT and control conditions), we evaluated induced responses by pathogen infection in LT-sensitive (ILC533) and -tolerant (Sel96Th11439) chickpea genotypes. In relation to genotype-specific defense reactions, tolerant plants responded more effective to the infection compared to sensitive plants, particularly after an acclimation treatment. LT treatments, and in particular an acclimation treatment, induced a significant decrease in disease symptoms 7 and 14 days after inoculation. Histopathological studies showed more infection throughout the cells of the sensitive genotype under control conditions. The minimal spread of the pathogen was observed in the tolerant genotype after acclimation treatment, and the infected plants did not contain pycnidia or hyphae of the pathogen. The electrolyte leakage index (ELI) and amount of malondialdehyde (MDA) after LT treatments were less than the values compared to post pathogen infection, indicating a difference in the degree of oxidative stress. Lower levels of pathogen-induced oxidative stress (ELI and MDA) and better photosynthetic efficiency (measured as F_v/F_m values) show that the tolerant genotype exposed to LT treatments acclimate better to the stress conditions compared to the sensitive genotype. Higher transcript levels for the superoxide dismutase, catalase and ascorbate peroxidase were detected during pathogen infection, particularly during the acclimation treatment, which might confirm lower oxidative stress in tolerant plants compared to sensitive plants. It is concluded that LT increased the capacity for tolerance to Ascochyta blight in chickpea plants and this was correlated with the stimulation of mechanisms involved in defense responses.

鹰嘴豆（Cicer arietinum）的两个主要胁迫是低温（LT）和Ascochyta叶枯病L.）栽培。暴露于LT处理（适应，LT，适应+ LT和对照条件）后，我们评估了对LT敏感（ILC533）和耐受（Sel96Th11439）鹰嘴豆基因型的病原体感染诱导的应答。关于基因型特异性防御​​反应，与敏感植物相比，耐性植物对感染的反应更为有效，尤其是在驯化处理后。LT疗法，特别是适应性疗法，在接种后7和14天引起疾病症状的显着降低。组织病理学研究显示，在控制条件下，敏感基因型的整个细胞感染更多。驯化处理后，在耐受的基因型中观察到病原体的最小扩散，并且被感染的植物不含病原体的菌丝或菌丝。LT处理后的电解质渗漏指数（ELI）和丙二醛（MDA）量小于病原体感染后的值，表明氧化应激程度存在差异。较低水平的病原体诱导的氧化应激（ELI和MDA）和更好的光合作用效率（按F _v / F _m值）表明，与敏感基因型相比，暴露于LT处理的耐受基因型更能适应压力条件。在病原体感染期间，尤其是在驯化处理期间，检测到较高的超氧化物歧化酶，过氧化氢酶和抗坏血酸过氧化物酶的转录水平，这可能证实与敏感植物相比，耐性植物的氧化应激较低。结论是，LT增强了鹰嘴豆植物对枯萎病的耐受能力，这与刺激参与防御反应的机制有关。