Abstract

The development of methods for long-term storage of microalgae remains an important and complex problem, the solution of which is impossible without studying the processes occurring during dehydration and rehydration of vegetative cells. The study presents the results from the investigation of changes in the dimensional characteristics of cells and trichomes of spirulina Arthrospira (Spirulina) platensis (Nordstedt) Gomont after dehydration and during reactivation (in dynamics) as well as processes occurring in them during reactivation. Methods close to natural dehydration were selected. Morphometric measurements of cells and trichomes of spirulina in their native state and during dehydration and rehydration were performed. Dehydration of A. platensis was accompanied by statistically significant changes in the morphometric characteristics of cells. For all methods of dehydration (in a thermostat at 30 and 60°C and on silica gel in a desiccator at a room temperature of 20°C) a decrease in their size, as well as elongation and flattening, were noted. The thickness and volume of trichomes after dehydration were reduced by two times. All processes occurring during reactivation were traced in dynamics, from the moment of moistening to the appearance of young threads. A relatively rapid flooding of the dried cells and a gradual restoration of their morphometric characteristics was noted. Among the reactivating trichomes, those in which the separation into viable and irreversibly areas damaged was clearly visible were found. Differential staining of trichomes with methylene blue revealed cells with strong plasmolysis inside externally intact threads. Morphometric parameters of the spirulina cell were restored within 24 h. This fact indicates that, in dehydrated cultures after rehydration, a number of complex biochemical processes occur, due to which the initial physiological state of the cells is restored.

中文翻译：

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脱水和活化期螺旋藻螺旋藻细胞和滴虫形态指标的变化。

摘要

微藻长期保存方法的发展仍然是一个重要而复杂的问题，如果不研究营养细胞在脱水和再水化过程中发生的过程，就不可能解决该问题。这项研究提出了调查螺旋藻节肢动物（螺旋藻）钝顶藻（Nordstedt）Gomont的细胞和毛状体的尺寸特征变化的结果，这些变化是在脱水过程中以及在活化过程中（动态）以及在活化过程中它们发生的过程。选择接近自然脱水的方法。螺旋藻的细胞和毛状体在其原始状态以及脱水和补液过程中的形态测量。脱水A.platensis伴随着细胞形态特征的统计学显着变化。对于所有的脱水方法（在30和60°C的恒温器中，以及在20°C的室温在干燥器中的硅胶上），它们的尺寸以及伸长率和展平性都减小了。脱水后毛线的厚度和体积减少了两倍。重新激活过程中发生的所有过程都从动力学开始，从润湿的时刻到年轻的线程出现。注意到干燥细胞的相对较快的淹没和其形态特征的逐渐恢复。在活化的毛线毛中，发现了清晰可见的分裂成可行和不可逆的受损区域的毛线毛。用亚甲蓝对毛状体进行差异染色，发现细胞在完整外线内具有强烈的胞浆裂解作用。螺旋藻细胞的形态参数在24小时内恢复。这一事实表明，在复水后的脱水培养物中，发生了许多复杂的生化过程，由于这些过程，细胞的初始生理状态得以恢复。