Abstract

The portable chlorophyll (Chl) meter SPAD-502 Plus (Konica Minolta Optics, Japan) is one of the most commonly used diagnostic tools for rapid and nondestructive estimation of Chl content in leaves of crops and tree species. The aim of this work was to evaluate the performance of a SPAD-502 Plus to estimate the content of Chl a, Chl b, and Chl (a + b) in Solanum lycopersicum L. leaves with mottled, interveinal chlorosis induced by continuous lighting. The results have shown positive correlation between the Chl content index (CCI) and contents per unit leaf area (mg/cm²) of Chl a (R² = 0.89), Chl b (R² = 0.79), Chl (a + b) (R² = 0.90) and between CCI and content per unit leaf mass (mg/g) of Chl a (R² = 0.88), Chl b (R² = 0.78), Chl (a + b) (R² = 0.89), suggesting that the portable Chl meter SPAD-502 Plus can be an effective tool for nondestructive estimation of Chl content in leaves with mottled, interveinal chlorosis. The accuracy of SPAD-502 Plus Chl meter tends to be higher for estimating Chl content per unit leaf area. Non-linear regressions were successfully used for estimating Chl content from SPAD measurements.

中文翻译：

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评估SPAD-502 Plus叶绿素仪以评估具有小叶绿化作用的叶片中叶绿素含量

摘要

便携式叶绿素（Chl）测量仪SPAD-502 Plus（日本柯尼卡美能达光学公司）是用于快速，无损估计农作物和树木物种中Chl含量的最常用诊断工具之一。这项工作的目的是评估一个SPAD-502的性能Plus来估计叶绿素的含量一，叶绿素b，和叶绿素（A + B）在番茄L.叶与由连续点亮诱导斑驳，脉间缺绿。结果表明，Chl含量指数（CCI）与Chl a（R ² = 0.89），Chl b（R的单位叶面积含量（mg / cm ²）正相关。² = 0.79），Chl（a + b）（R ²  = 0.90）和CCI与Chl a的每片叶子质量含量（mg / g）之间（R ² = 0.88），Chl b（R ² = 0.78）， Chl（a + b）（R ²  = 0.89），表明便携式Chl测量仪SPAD-502 Plus可以有效地用于斑驳，小叶萎黄的叶片中Chl含量的无损估计。SPAD-502 Plus Chl计的精度往往更高，以估计每单位叶面积的Chl含量。非线性回归已成功用于从SPAD测量中估计Chl含量。