Abstract

The process of extraction of the extracellular matrix (ECM) requires operational monitoring, since there is a risk of destruction of the main proteins of the cell matrix at non-optimal parameters of the process. Using multiphoton microscopy the structure of collagen, the integrity of the cell membrane and the content of elastin in the process of isolation of ECM from various tissues of the rat body (brain, dermis, and muscle) has been studied. It has been shown that multiphoton microscopy is an effective tool for monitoring the quality of ECM extraction.

中文翻译：

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多光子激发显微镜用于身体组织细胞外基质成分的识别和操作控制

摘要

提取细胞外基质（ECM）的过程需要进行操作监控，因为在该过程的非最佳参数下存在破坏细胞基质主要蛋白的风险。使用多光子显微镜研究了从大鼠体内各种组织（大脑，真皮和肌肉）分离ECM的过程中胶原蛋白的结构，细胞膜的完整性和弹性蛋白的含量。已经表明，多光子显微镜术是监测ECM提取质量的有效工具。